7d55
From Proteopedia
(Difference between revisions)
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==Crystal structure of lys170 CBD== | ==Crystal structure of lys170 CBD== | ||
| - | <StructureSection load='7d55' size='340' side='right'caption='[[7d55]]' scene=''> | + | <StructureSection load='7d55' size='340' side='right'caption='[[7d55]], [[Resolution|resolution]] 1.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D55 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7d55]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_phage_phim1ef22 Enterococcus phage phim1ef22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D55 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d55 OCA], [https://pdbe.org/7d55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d55 RCSB], [https://www.ebi.ac.uk/pdbsum/7d55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d55 ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PHIM1EF22_0110 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2218498 Enterococcus phage phiM1EF22])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d55 OCA], [https://pdbe.org/7d55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d55 RCSB], [https://www.ebi.ac.uk/pdbsum/7d55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d55 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lysins are a class of hydrolytic enzymes used by bacteriophages to target and cleave the peptidoglycan of bacterial cell walls during their lytic cycle. The lysins from bacteriophages that infect Gram-positive bacteria are typically monomeric and consist of one or two catalytic domains (CD) and a cell binding domain (CBD). However, multimeric lysins encoded by a single gene have also been reported, among which Lys170 from enterococcal phage F170/08 was one of the first identified. Here, we determined the crystal structure of Lys170 CBD at 1.40 A resolution. The structure reveals that Lys170 CBDs assemble into a tetrameric functional unit and that each monomer folds into a three-stranded beta-sheet core capped on each side by an alpha-helix. In addition, we identified key residues of Lys170 CBD involved in host cell binding. Our work provides a basis for designing highly efficient lysins targeting Enterococcus faecalis. | ||
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| + | Structural and biochemical analyses of the tetrameric cell binding domain of Lys170 from enterococcal phage F170/08.,Xu X, Zhang D, Zhou B, Zhen X, Ouyang S Eur Biophys J. 2021 Feb 20. pii: 10.1007/s00249-021-01511-x. doi:, 10.1007/s00249-021-01511-x. PMID:33609147<ref>PMID:33609147</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7d55" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Enterococcus phage phim1ef22]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Zhen X]] | + | [[Category: Zhen, X]] |
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Lysin]] | ||
Revision as of 10:06, 14 July 2021
Crystal structure of lys170 CBD
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