7l7v
From Proteopedia
(Difference between revisions)
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==Crystal structure of Arabidopsis NRG1.1 CC-R domain K94E/K96E/R99E/K100E/R103E/K106E/K110E mutant== | ==Crystal structure of Arabidopsis NRG1.1 CC-R domain K94E/K96E/R99E/K100E/R103E/K106E/K110E mutant== | ||
| - | <StructureSection load='7l7v' size='340' side='right'caption='[[7l7v]]' scene=''> | + | <StructureSection load='7l7v' size='340' side='right'caption='[[7l7v]], [[Resolution|resolution]] 2.95Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L7V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7l7v]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L7V FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l7v OCA], [https://pdbe.org/7l7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l7v RCSB], [https://www.ebi.ac.uk/pdbsum/7l7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l7v ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At5g66900, MUD21.16 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l7v OCA], [https://pdbe.org/7l7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l7v RCSB], [https://www.ebi.ac.uk/pdbsum/7l7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l7v ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/DRL42_ARATH DRL42_ARATH]] Probable disease resistance protein. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In Arabidopsis, a subfamily of "helper" NLRs are required by many "sensor" NLRs. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta and conferred cytoplasmic Ca(2+) influx in plant and human cells. NRG1.1-dependent Ca(2+) influx and cell death were sensitive to Ca(2+) channel blockers and were suppressed by mutations impacting oligomerization or plasma membrane enrichment. Ca(2+) influx and cell death mediated by NRG1.1 and ACTIVATED DISEASE RESISTANCE 1 (ADR1), another "helper" NLR, required conserved negatively charged N-terminal residues. Whole-cell voltage-clamp recordings demonstrate that Arabidopsis "helper" NLRs form Ca(2+)-permeable cation channels to directly regulate cytoplasmic Ca(2+) levels and consequent cell death. Thus, "helper" NLRs transduce cell death signals directly. | ||
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| + | Plant "helper" immune receptors are Ca(2+)-permeable nonselective cation channels.,Jacob P, Kim NH, Wu F, El-Kasmi F, Chi Y, Walton WG, Furzer OJ, Lietzan AD, Sunil S, Kempthorn K, Redinbo MR, Pei ZM, Wan L, Dangl JL Science. 2021 Jun 17. pii: science.abg7917. doi: 10.1126/science.abg7917. PMID:34140391<ref>PMID:34140391</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7l7v" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Arath]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Dangl | + | [[Category: Dangl, J L]] |
| - | [[Category: Lietzan | + | [[Category: Lietzan, A D]] |
| - | [[Category: Redinbo | + | [[Category: Redinbo, M R]] |
| - | [[Category: Walton | + | [[Category: Walton, W G]] |
| - | [[Category: Wan L]] | + | [[Category: Wan, L]] |
| + | [[Category: 4-helix bundle]] | ||
| + | [[Category: Calcium channel]] | ||
| + | [[Category: Cell death]] | ||
| + | [[Category: Immune system]] | ||
| + | [[Category: Membrane pore]] | ||
Revision as of 10:10, 14 July 2021
Crystal structure of Arabidopsis NRG1.1 CC-R domain K94E/K96E/R99E/K100E/R103E/K106E/K110E mutant
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