1atn

From Proteopedia

(Difference between revisions)

Revision as of 10:27, 14 July 2021

Atomic structure of the actin:DNASE I complex

Structural highlights

1atn is a 2 chain structure with sequence from Bos taurus and Oryctolagus cuniculus. The June 2001 RCSB PDB Molecule of the Month feature on Myosin by David S. Goodsell is 10.2210/rcsb_pdb/mom_2001_6. The July 2001 RCSB PDB Molecule of the Month feature on Actin by David S. Goodsell is 10.2210/rcsb_pdb/mom_2001_7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
NonStd Res:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[ACTS_HUMAN] Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8] ^[9] ^[10] Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.^[11] Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.^[12] ^[13]

Function

[ACTS_HUMAN] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [DNAS1_BOVIN] Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The atomic models of the complex between rabbit skeletal muscle actin and bovine pancreatic deoxyribonuclease I both in the ATP and ADP forms have been determined by X-ray analysis at an effective resolution of 2.8 A and 3A, respectively. The two structures are very similar. The actin molecule consists of two domains which can be further subdivided into two subdomains. ADP or ATP is located in the cleft between the domains with a calcium ion bound to the beta- or beta- and gamma-phosphates, respectively. The motif of a five-stranded beta sheet consisting of a beta meander and a right handed beta alpha beta unit appears in each domain suggesting that gene duplication might have occurred. These sheets have the same topology as that found in hexokinase.

Atomic structure of the actin:DNase I complex.,Kabsch W, Mannherz HG, Suck D, Pai EF, Holmes KC Nature. 1990 Sep 6;347(6288):37-44. PMID:2395459^[14]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nowak KJ, Wattanasirichaigoon D, Goebel HH, Wilce M, Pelin K, Donner K, Jacob RL, Hubner C, Oexle K, Anderson JR, Verity CM, North KN, Iannaccone ST, Muller CR, Nurnberg P, Muntoni F, Sewry C, Hughes I, Sutphen R, Lacson AG, Swoboda KJ, Vigneron J, Wallgren-Pettersson C, Beggs AH, Laing NG. Mutations in the skeletal muscle alpha-actin gene in patients with actin myopathy and nemaline myopathy. Nat Genet. 1999 Oct;23(2):208-12. PMID:10508519 doi:10.1038/13837
↑ Ilkovski B, Cooper ST, Nowak K, Ryan MM, Yang N, Schnell C, Durling HJ, Roddick LG, Wilkinson I, Kornberg AJ, Collins KJ, Wallace G, Gunning P, Hardeman EC, Laing NG, North KN. Nemaline myopathy caused by mutations in the muscle alpha-skeletal-actin gene. Am J Hum Genet. 2001 Jun;68(6):1333-43. Epub 2001 Apr 27. PMID:11333380 doi:S0002-9297(07)61044-1
↑ Jungbluth H, Sewry CA, Brown SC, Nowak KJ, Laing NG, Wallgren-Pettersson C, Pelin K, Manzur AY, Mercuri E, Dubowitz V, Muntoni F. Mild phenotype of nemaline myopathy with sleep hypoventilation due to a mutation in the skeletal muscle alpha-actin (ACTA1) gene. Neuromuscul Disord. 2001 Jan;11(1):35-40. PMID:11166164
↑ Agrawal PB, Strickland CD, Midgett C, Morales A, Newburger DE, Poulos MA, Tomczak KK, Ryan MM, Iannaccone ST, Crawford TO, Laing NG, Beggs AH. Heterogeneity of nemaline myopathy cases with skeletal muscle alpha-actin gene mutations. Ann Neurol. 2004 Jul;56(1):86-96. PMID:15236405 doi:10.1002/ana.20157
↑ Ilkovski B, Nowak KJ, Domazetovska A, Maxwell AL, Clement S, Davies KE, Laing NG, North KN, Cooper ST. Evidence for a dominant-negative effect in ACTA1 nemaline myopathy caused by abnormal folding, aggregation and altered polymerization of mutant actin isoforms. Hum Mol Genet. 2004 Aug 15;13(16):1727-43. Epub 2004 Jun 15. PMID:15198992 doi:10.1093/hmg/ddh185
↑ Kaindl AM, Ruschendorf F, Krause S, Goebel HH, Koehler K, Becker C, Pongratz D, Muller-Hocker J, Nurnberg P, Stoltenburg-Didinger G, Lochmuller H, Huebner A. Missense mutations of ACTA1 cause dominant congenital myopathy with cores. J Med Genet. 2004 Nov;41(11):842-8. PMID:15520409 doi:10.1136/jmg.2004.020271
↑ Ohlsson M, Tajsharghi H, Darin N, Kyllerman M, Oldfors A. Follow-up of nemaline myopathy in two patients with novel mutations in the skeletal muscle alpha-actin gene (ACTA1). Neuromuscul Disord. 2004 Sep;14(8-9):471-5. PMID:15336687 doi:10.1016/j.nmd.2004.05.016
↑ Hutchinson DO, Charlton A, Laing NG, Ilkovski B, North KN. Autosomal dominant nemaline myopathy with intranuclear rods due to mutation of the skeletal muscle ACTA1 gene: clinical and pathological variability within a kindred. Neuromuscul Disord. 2006 Feb;16(2):113-21. Epub 2006 Jan 19. PMID:16427282 doi:10.1016/j.nmd.2005.11.004
↑ D'Amico A, Graziano C, Pacileo G, Petrini S, Nowak KJ, Boldrini R, Jacques A, Feng JJ, Porfirio B, Sewry CA, Santorelli FM, Limongelli G, Bertini E, Laing N, Marston SB. Fatal hypertrophic cardiomyopathy and nemaline myopathy associated with ACTA1 K336E mutation. Neuromuscul Disord. 2006 Oct;16(9-10):548-52. Epub 2006 Sep 1. PMID:16945537 doi:10.1016/j.nmd.2006.07.005
↑ Domazetovska A, Ilkovski B, Kumar V, Valova VA, Vandebrouck A, Hutchinson DO, Robinson PJ, Cooper ST, Sparrow JC, Peckham M, North KN. Intranuclear rod myopathy: molecular pathogenesis and mechanisms of weakness. Ann Neurol. 2007 Dec;62(6):597-608. PMID:17705262 doi:10.1002/ana.21200
↑ Nowak KJ, Wattanasirichaigoon D, Goebel HH, Wilce M, Pelin K, Donner K, Jacob RL, Hubner C, Oexle K, Anderson JR, Verity CM, North KN, Iannaccone ST, Muller CR, Nurnberg P, Muntoni F, Sewry C, Hughes I, Sutphen R, Lacson AG, Swoboda KJ, Vigneron J, Wallgren-Pettersson C, Beggs AH, Laing NG. Mutations in the skeletal muscle alpha-actin gene in patients with actin myopathy and nemaline myopathy. Nat Genet. 1999 Oct;23(2):208-12. PMID:10508519 doi:10.1038/13837
↑ Laing NG, Clarke NF, Dye DE, Liyanage K, Walker KR, Kobayashi Y, Shimakawa S, Hagiwara T, Ouvrier R, Sparrow JC, Nishino I, North KN, Nonaka I. Actin mutations are one cause of congenital fibre type disproportion. Ann Neurol. 2004 Nov;56(5):689-94. PMID:15468086 doi:10.1002/ana.20260
↑ Clarke NF, Ilkovski B, Cooper S, Valova VA, Robinson PJ, Nonaka I, Feng JJ, Marston S, North K. The pathogenesis of ACTA1-related congenital fiber type disproportion. Ann Neurol. 2007 Jun;61(6):552-61. PMID:17387733 doi:10.1002/ana.21112
↑ Kabsch W, Mannherz HG, Suck D, Pai EF, Holmes KC. Atomic structure of the actin:DNase I complex. Nature. 1990 Sep 6;347(6288):37-44. PMID:2395459 doi:http://dx.doi.org/10.1038/347037a0

1 Structural highlights
2 Disease
3 Function
4 Evolutionary Conservation
5 Publication Abstract from PubMed
6 See Also
7 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1atn"

@@ Line 3: / Line 3: @@
 <StructureSection load='1atn' size='340' side='right'caption='[[1atn]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1atn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. The June 2001 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Myosin''  by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2001_6 10.2210/rcsb_pdb/mom_2001_6]. The July 2001 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Actin''  by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2001_7 10.2210/rcsb_pdb/mom_2001_7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ATN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ATN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1atn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. The June 2001 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Myosin''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2001_6 10.2210/rcsb_pdb/mom_2001_6]. The July 2001 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Actin''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2001_7 10.2210/rcsb_pdb/mom_2001_7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ATN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ATN FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1atn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1atn OCA], [http://pdbe.org/1atn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1atn RCSB], [http://www.ebi.ac.uk/pdbsum/1atn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1atn ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1atn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1atn OCA], [https://pdbe.org/1atn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1atn RCSB], [https://www.ebi.ac.uk/pdbsum/1atn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1atn ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/ACTS_HUMAN ACTS_HUMAN]] Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:[http://omim.org/entry/161800 161800]]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.<ref>PMID:10508519</ref> <ref>PMID:11333380</ref> <ref>PMID:11166164</ref> <ref>PMID:15236405</ref> <ref>PMID:15198992</ref> <ref>PMID:15520409</ref> <ref>PMID:15336687</ref> <ref>PMID:16427282</ref> <ref>PMID:16945537</ref> <ref>PMID:17705262</ref>   Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:[http://omim.org/entry/161800 161800]]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.<ref>PMID:10508519</ref>   Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:[http://omim.org/entry/255310 255310]]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.<ref>PMID:15468086</ref> <ref>PMID:17387733</ref>
+[[https://www.uniprot.org/uniprot/ACTS_HUMAN ACTS_HUMAN]] Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:[https://omim.org/entry/161800 161800]]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.<ref>PMID:10508519</ref> <ref>PMID:11333380</ref> <ref>PMID:11166164</ref> <ref>PMID:15236405</ref> <ref>PMID:15198992</ref> <ref>PMID:15520409</ref> <ref>PMID:15336687</ref> <ref>PMID:16427282</ref> <ref>PMID:16945537</ref> <ref>PMID:17705262</ref>   Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:[https://omim.org/entry/161800 161800]]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.<ref>PMID:10508519</ref>   Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:[https://omim.org/entry/255310 255310]]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.<ref>PMID:15468086</ref> <ref>PMID:17387733</ref>
 == Function ==
-[[http://www.uniprot.org/uniprot/ACTS_HUMAN ACTS_HUMAN]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [[http://www.uniprot.org/uniprot/DNAS1_BOVIN DNAS1_BOVIN]] Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).
+[[https://www.uniprot.org/uniprot/ACTS_HUMAN ACTS_HUMAN]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [[https://www.uniprot.org/uniprot/DNAS1_BOVIN DNAS1_BOVIN]] Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 34: / Line 34: @@
 ==See Also==
 *[[Actin 3D structures|Actin 3D structures]]
-*[[Non-polymerizable monomeric actin|Non-polymerizable monomeric actin]]
 == References ==
 <references/>

1atn

From Proteopedia

Revision as of 10:27, 14 July 2021

Atomic structure of the actin:DNASE I complex

Structural highlights

Disease

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

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Proteopedia Page Contributors and Editors (what is this?)

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