1cq4

From Proteopedia

(Difference between revisions)

Revision as of 10:39, 14 July 2021

CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59

Structural highlights

1cq4 is a 2 chain structure with sequence from Barley. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ICI2_HORVU] Inhibits both subtilisin and chymotrypsin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We have constructed mutants of chymotrypsin inhibitor 2 with short glutamine repeats inserted into its inhibitory loop. These mutants oligomerize when expressed in Escherichia coli. The dimer of a mutant with four glutamines now has been crystallized, and its structure has been solved by molecular replacement by using the wild-type monomer as a search model. The structure of each half of the dimer is found to be the same as that of the wild-type monomer, except around the glutamine insertion. It was proposed that the components of the oligomers are held together by hydrogen bonds between the main-chain and side-chain amides of the glutamine repeats. Instead, they appear to form by swapping domains on folding in E. coli, and the glutamine repeats connecting the components of the dimers are disordered.

Crystal structure of a dimeric chymotrypsin inhibitor 2 mutant containing an inserted glutamine repeat.,Chen YW, Stott K, Perutz MF Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1257-61. PMID:9990011^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chen YW, Stott K, Perutz MF. Crystal structure of a dimeric chymotrypsin inhibitor 2 mutant containing an inserted glutamine repeat. Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1257-61. PMID:9990011

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1cq4"

@@ Line 3: / Line 3: @@
 <StructureSection load='1cq4' size='340' side='right'caption='[[1cq4]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1cq4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Barley Barley]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CQ4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1cq4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Barley Barley]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CQ4 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cq4 OCA], [http://pdbe.org/1cq4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cq4 RCSB], [http://www.ebi.ac.uk/pdbsum/1cq4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cq4 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cq4 OCA], [https://pdbe.org/1cq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cq4 RCSB], [https://www.ebi.ac.uk/pdbsum/1cq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cq4 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ICI2_HORVU ICI2_HORVU]] Inhibits both subtilisin and chymotrypsin.
+[[https://www.uniprot.org/uniprot/ICI2_HORVU ICI2_HORVU]] Inhibits both subtilisin and chymotrypsin.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

1cq4

From Proteopedia

Revision as of 10:39, 14 July 2021

CI2 MUTANT WITH TETRAGLUTAMINE (MGQQQQGM) REPLACING MET59

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox