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1ev7

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[[Image:1ev7.gif|left|200px]]
[[Image:1ev7.gif|left|200px]]
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{{Structure
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|PDB= 1ev7 |SIZE=350|CAPTION= <scene name='initialview01'>1ev7</scene>, resolution 2.38&Aring;
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The line below this paragraph, containing "STRUCTURE_1ev7", creates the "Structure Box" on the page.
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
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{{STRUCTURE_1ev7| PDB=1ev7 | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ev7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ev7 OCA], [http://www.ebi.ac.uk/pdbsum/1ev7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ev7 RCSB]</span>
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'''CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI'''
'''CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI'''
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[[Category: Luo, F.]]
[[Category: Luo, F.]]
[[Category: Zhao, Y.]]
[[Category: Zhao, Y.]]
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[[Category: apo-naei]]
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[[Category: Apo-naei]]
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[[Category: cap]]
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[[Category: Cap]]
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[[Category: helix-turn-helix]]
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[[Category: Helix-turn-helix]]
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[[Category: restriction endonuclease]]
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[[Category: Restriction endonuclease]]
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[[Category: topoisomerase]]
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[[Category: Topoisomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:10:15 2008''
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Revision as of 12:33, 2 May 2008

Image:1ev7.gif

Template:STRUCTURE 1ev7

CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI


Overview

NAE:I is transformed from DNA endonuclease to DNA topoisomerase and recombinase by a single amino acid substitution. The crystal structure of NAE:I was solved at 2.3 A resolution and shows that NAE:I is a dimeric molecule with two domains per monomer. Each domain contains one potential DNA recognition motif corresponding to either endonuclease or topoisomerase activity. The N-terminal domain core folds like the other type II restriction endonucleases as well as lambda-exonuclease and the DNA repair enzymes MutH and Vsr, implying a common evolutionary origin and catalytic mechanism. The C-terminal domain contains a catabolite activator protein (CAP) motif present in many DNA-binding proteins, including the type IA and type II topoisomerases. Thus, the NAE:I structure implies that DNA processing enzymes evolved from a few common ancestors. NAE:I may be an evolutionary bridge between endonuclease and DNA processing enzymes.

About this Structure

1EV7 is a Single protein structure of sequence from Lechevalieria aerocolonigenes. Full crystallographic information is available from OCA.

Reference

Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase., Huai Q, Colandene JD, Chen Y, Luo F, Zhao Y, Topal MD, Ke H, EMBO J. 2000 Jun 15;19(12):3110-8. PMID:10856254 Page seeded by OCA on Fri May 2 15:33:23 2008

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