1ew3
From Proteopedia
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'''CRYSTAL STRUCTURE OF THE MAJOR HORSE ALLERGEN EQU C 1''' | '''CRYSTAL STRUCTURE OF THE MAJOR HORSE ALLERGEN EQU C 1''' | ||
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[[Category: Rosinski-Chupin, I.]] | [[Category: Rosinski-Chupin, I.]] | ||
[[Category: Tavares, G A.]] | [[Category: Tavares, G A.]] | ||
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| - | [[Category: | + | [[Category: Lipocalin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:35:19 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:35, 2 May 2008
CRYSTAL STRUCTURE OF THE MAJOR HORSE ALLERGEN EQU C 1
Overview
The three-dimensional structure of the major horse allergen Equ c 1 has been determined at 2.3 A resolution by x-ray crystallography. Equ c 1 displays the typical fold of lipocalins, a beta-barrel flanked by a C-terminal alpha-helix. The space between the two beta-sheets of the barrel defines an internal cavity that could serve, as in other lipocalins, for the binding and transport of small hydrophobic ligands. Equ c 1 crystallizes in a novel dimeric form, which is distinct from that observed in other lipocalin dimers and corresponds to the functional form of the allergen. Binding studies of point mutants of the allergen with specific monoclonal antibodies raised in mouse and IgE serum from horse allergic patients allowed to identify putative B cell antigenic determinants. In addition, total inhibition of IgE serum recognition by a single specific monoclonal antibody revealed the restricted nature of the IgE binding target on the molecular surface of Equ c 1.
About this Structure
1EW3 is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the allergen Equ c 1. A dimeric lipocalin with restricted IgE-reactive epitopes., Lascombe MB, Gregoire C, Poncet P, Tavares GA, Rosinski-Chupin I, Rabillon J, Goubran-Botros H, Mazie JC, David B, Alzari PM, J Biol Chem. 2000 Jul 14;275(28):21572-7. PMID:10787420 Page seeded by OCA on Fri May 2 15:35:19 2008
