1exn
From Proteopedia
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[[Image:1exn.jpg|left|200px]] | [[Image:1exn.jpg|left|200px]] | ||
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'''T5 5'-EXONUCLEASE''' | '''T5 5'-EXONUCLEASE''' | ||
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A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease., Ceska TA, Sayers JR, Stier G, Suck D, Nature. 1996 Jul 4;382(6586):90-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8657312 8657312] | A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease., Ceska TA, Sayers JR, Stier G, Suck D, Nature. 1996 Jul 4;382(6586):90-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8657312 8657312] | ||
[[Category: Enterobacteria phage t5]] | [[Category: Enterobacteria phage t5]] | ||
| - | [[Category: Exodeoxyribonuclease (lambda-induced)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ceska, T A.]] | [[Category: Ceska, T A.]] | ||
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[[Category: Stier, G.]] | [[Category: Stier, G.]] | ||
[[Category: Suck, D.]] | [[Category: Suck, D.]] | ||
| - | [[Category: | + | [[Category: Exonuclease]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Nuclease]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:38:36 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:38, 2 May 2008
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| 1exn, resolution 2.50Å () | |||||||||
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| Non-Standard Residues: | |||||||||
| Gene: | D15 (Enterobacteria phage T5) | ||||||||
| Activity: | Exodeoxyribonuclease (lambda-induced), with EC number 3.1.11.3 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
T5 5'-EXONUCLEASE
Overview
THE 5'-exonucleases are enzymes that are essential for DNA replication and repair. As well as their exonucleolytic action, removing nucleotides from the 5'-end of nucleic acid molecules such as Okazaki fragments, many 5'-3'-exonucleases have been shown to possess endonucleolytic activities. T5 5'-3'-exonuclease shares many similarities with the amino terminal of eubacterial DNA polymerases, although, unlike eubacteria, phages such as T5, T4 and T7 express polymerase and 5'-exonuclease proteins from separate genes. Here we report the 2.5-A crystal structure of the phage T5 5'-exonuclease, which reveals a helical arch for binding DNA. We propose a model consistent with a threading mechanism in which single-stranded DNA could slide through the arch, which is formed by two helices, one containing positively charged, and the other hydrophobic, residues. The active site is at the base of the arch, and contains two metal-binding sites.
About this Structure
1EXN is a Single protein structure of sequence from Enterobacteria phage t5. Full crystallographic information is available from OCA.
Reference
A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease., Ceska TA, Sayers JR, Stier G, Suck D, Nature. 1996 Jul 4;382(6586):90-3. PMID:8657312 Page seeded by OCA on Fri May 2 15:38:36 2008
