6zni
From Proteopedia
(Difference between revisions)
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==Structure of the Shigella MxiH needle filament attached to the basal body== | ==Structure of the Shigella MxiH needle filament attached to the basal body== | ||
| - | <StructureSection load='6zni' size='340' side='right'caption='[[6zni]]' scene=''> | + | <StructureSection load='6zni' size='340' side='right'caption='[[6zni]], [[Resolution|resolution]] 3.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZNI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6zni]] is a 23 chain structure with sequence from [https://en.wikipedia.org/wiki/Shifm Shifm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZNI FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zni OCA], [https://pdbe.org/6zni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zni RCSB], [https://www.ebi.ac.uk/pdbsum/6zni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zni ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mxiH, CP0137 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1086030 SHIFM])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zni OCA], [https://pdbe.org/6zni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zni RCSB], [https://www.ebi.ac.uk/pdbsum/6zni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zni ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/MXIH_SHIFL MXIH_SHIFL]] Necessary for the secretion of IPA invasins. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of Salmonella PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development. | ||
| + | |||
| + | Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies.,Kotov V, Lunelli M, Wald J, Kolbe M, Marlovits TC Biochem Biophys Rep. 2021 Jun 27;27:101039. doi: 10.1016/j.bbrep.2021.101039., eCollection 2021 Sep. PMID:34258394<ref>PMID:34258394</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6zni" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Kolbe M]] | + | [[Category: Shifm]] |
| - | [[Category: Kotov V]] | + | [[Category: Kolbe, M]] |
| - | [[Category: Lunelli M]] | + | [[Category: Kotov, V]] |
| - | [[Category: Marlovits | + | [[Category: Lunelli, M]] |
| + | [[Category: Marlovits, T C]] | ||
| + | [[Category: Helical reconstruction]] | ||
| + | [[Category: Needle filament]] | ||
| + | [[Category: Protein transport]] | ||
| + | [[Category: Shigella]] | ||
| + | [[Category: Type 3 secretion system]] | ||
Revision as of 10:44, 28 July 2021
Structure of the Shigella MxiH needle filament attached to the basal body
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