7f1t
From Proteopedia
(Difference between revisions)
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==Crystal structure of the human chemokine receptor CCR5 in complex with MIP-1a== | ==Crystal structure of the human chemokine receptor CCR5 in complex with MIP-1a== | ||
| - | <StructureSection load='7f1t' size='340' side='right'caption='[[7f1t]]' scene=''> | + | <StructureSection load='7f1t' size='340' side='right'caption='[[7f1t]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F1T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7f1t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_pasteurianus"_(winogradsky_1895)_lehmann_and_neumann_1907 "bacillus pasteurianus" (winogradsky 1895) lehmann and neumann 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F1T FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f1t OCA], [https://pdbe.org/7f1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f1t RCSB], [https://www.ebi.ac.uk/pdbsum/7f1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f1t ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CCR5, CMKBR5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1501 "Bacillus pasteurianus" (Winogradsky 1895) Lehmann and Neumann 1907])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f1t OCA], [https://pdbe.org/7f1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f1t RCSB], [https://www.ebi.ac.uk/pdbsum/7f1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f1t ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/CCL3_HUMAN CCL3_HUMAN]] Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).<ref>PMID:8525373</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The chemokine receptor CCR5 plays a vital role in immune surveillance and inflammation. However, molecular details that govern its endogenous chemokine recognition and receptor activation remain elusive. Here we report three cryo-electron microscopy structures of Gi1 protein-coupled CCR5 in a ligand-free state and in complex with the chemokine MIP-1alpha or RANTES, as well as the crystal structure of MIP-1alpha-bound CCR5. These structures reveal distinct binding modes of the two chemokines and a specific accommodate pattern of the chemokine for the distal N terminus of CCR5. Together with functional data, the structures demonstrate that chemokine-induced rearrangement of toggle switch and plasticity of the receptor extracellular region are critical for receptor activation, while a conserved tryptophan residue in helix II acts as a trigger of receptor constitutive activation. | ||
| + | |||
| + | Structural basis for chemokine recognition and receptor activation of chemokine receptor CCR5.,Zhang H, Chen K, Tan Q, Shao Q, Han S, Zhang C, Yi C, Chu X, Zhu Y, Xu Y, Zhao Q, Wu B Nat Commun. 2021 Jul 6;12(1):4151. doi: 10.1038/s41467-021-24438-5. PMID:34230484<ref>PMID:34230484</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7f1t" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Chen K]] | + | [[Category: Chen, K]] |
| - | [[Category: Han S]] | + | [[Category: Han, S]] |
| - | [[Category: Tan Q]] | + | [[Category: Tan, Q]] |
| - | [[Category: Wu B]] | + | [[Category: Wu, B]] |
| - | [[Category: Zhang H]] | + | [[Category: Zhang, H]] |
| - | [[Category: Zhao Q]] | + | [[Category: Zhao, Q]] |
| - | [[Category: Zhu Y]] | + | [[Category: Zhu, Y]] |
| + | [[Category: Chemokine receptor ccr5]] | ||
| + | [[Category: G protein-coupled receptor]] | ||
| + | [[Category: Mip-1a]] | ||
| + | [[Category: Signaling protein]] | ||
Revision as of 10:48, 28 July 2021
Crystal structure of the human chemokine receptor CCR5 in complex with MIP-1a
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