7nm7
From Proteopedia
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==The crystal structure of the antimycin pathway standalone ketoreductase, AntM== | ==The crystal structure of the antimycin pathway standalone ketoreductase, AntM== | ||
| - | <StructureSection load='7nm7' size='340' side='right'caption='[[7nm7]]' scene=''> | + | <StructureSection load='7nm7' size='340' side='right'caption='[[7nm7]], [[Resolution|resolution]] 2.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NM7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NM7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7nm7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_gibsoni"_(sic)_dodge_1935 "actinomyces gibsoni" (sic) dodge 1935]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NM7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NM7 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nm7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nm7 OCA], [https://pdbe.org/7nm7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nm7 RCSB], [https://www.ebi.ac.uk/pdbsum/7nm7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nm7 ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">antM ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1888 "Actinomyces Gibsoni" (sic) Dodge 1935])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nm7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nm7 OCA], [https://pdbe.org/7nm7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nm7 RCSB], [https://www.ebi.ac.uk/pdbsum/7nm7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nm7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Antimycins are anticancer compounds produced by a hybrid nonribosomal peptide synthetase/polyketide synthase (NRPS/PKS) pathway. The biosynthesis of these compounds is well characterized, with the exception of the standalone beta-ketoreductase enzyme AntM that is proposed to catalyze the reduction of the C8 carbonyl of the antimycin scaffold. Inactivation of antM and structural characterization suggested that rather than functioning as a post-PKS tailoring enzyme, AntM acts upon the terminal biosynthetic intermediate while it is tethered to the PKS acyl carrier protein. Mutational analysis identified two amino acid residues (Tyr185 and Phe223) that are proposed to serve as checkpoints controlling substrate access to the AntM active site. Aromatic checkpoint residues are conserved in uncharacterized standalone beta-ketoreductases, indicating that they may also act concomitantly with synthesis of the scaffold. These data provide novel mechanistic insights into the functionality of standalone beta-ketoreductases and will enable their reprogramming for combinatorial biosynthesis. | ||
| + | |||
| + | Standalone beta-Ketoreductase Acts Concomitantly with Biosynthesis of the Antimycin Scaffold.,Fazal A, Hemsworth GR, Webb ME, Seipke RF ACS Chem Biol. 2021 Jun 20. doi: 10.1021/acschembio.1c00229. PMID:34151573<ref>PMID:34151573</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7nm7" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Fazal A]] | + | [[Category: Fazal, A]] |
| - | [[Category: Hemsworth | + | [[Category: Hemsworth, G R]] |
| - | [[Category: Seipke | + | [[Category: Seipke, R F]] |
| - | [[Category: Webb | + | [[Category: Webb, M E]] |
| + | [[Category: Antimycin]] | ||
| + | [[Category: Ketoreductase]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: Polyketide]] | ||
| + | [[Category: Standalone]] | ||
Revision as of 10:58, 28 July 2021
The crystal structure of the antimycin pathway standalone ketoreductase, AntM
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