1g60

From Proteopedia

(Difference between revisions)

Revision as of 11:19, 28 July 2021

Crystal Structure of Methyltransferase MboIIa (Moraxella bovis)

Structural highlights

1g60 is a 2 chain structure with sequence from "haemophilus_bovis"_hauduroy_et_al._1937 "haemophilus bovis" hauduroy et al. 1937. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Activity:	Site-specific DNA-methyltransferase (adenine-specific), with EC number 2.1.1.72
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

[MTM2_MORBO] This methylase recognizes the double-stranded sequence GAAGA, causes specific methylation on A-5, and protects the DNA from cleavage by the MboII endonuclease. It is not known if the cytosine of the complementary sequence TCTTC is also methylated by this enzyme.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

DNA methyltransferases (MTases) are sequence-specific enzymes which transfer a methyl group from S-adenosyl-L-methionine (AdoMet) to the amino group of either cytosine or adenine within a recognized DNA sequence. Methylation of a base in a specific DNA sequence protects DNA from nucleolytic cleavage by restriction enzymes recognizing the same DNA sequence. We have determined at 1.74 A resolution the crystal structure of a beta-class DNA MTase MboIIA (M.MboIIA) from the bacterium Moraxella bovis, the smallest DNA MTase determined to date. M.MboIIA methylates the 3' adenine of the pentanucleotide sequence 5'-GAAGA-3'. The protein crystallizes with two molecules in the asymmetric unit which we propose to resemble the dimer when M.MboIIA is not bound to DNA. The overall structure of the enzyme closely resembles that of M.RsrI. However, the cofactor-binding pocket in M.MboIIA forms a closed structure which is in contrast to the open-form structures of other known MTases.

Crystal structure of MboIIA methyltransferase.,Osipiuk J, Walsh MA, Joachimiak A Nucleic Acids Res. 2003 Sep 15;31(18):5440-8. PMID:12954781^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Osipiuk J, Walsh MA, Joachimiak A. Crystal structure of MboIIA methyltransferase. Nucleic Acids Res. 2003 Sep 15;31(18):5440-8. PMID:12954781

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1g60"

@@ Line 3: / Line 3: @@
 <StructureSection load='1g60' size='340' side='right'caption='[[1g60]], [[Resolution|resolution]] 1.74&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1g60]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"haemophilus_bovis"_hauduroy_et_al._1937 "haemophilus bovis" hauduroy et al. 1937]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G60 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G60 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1g60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"haemophilus_bovis"_hauduroy_et_al._1937 "haemophilus bovis" hauduroy et al. 1937]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G60 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] </span></td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g60 OCA], [http://pdbe.org/1g60 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g60 RCSB], [http://www.ebi.ac.uk/pdbsum/1g60 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1g60 ProSAT], [http://www.topsan.org/Proteins/MCSG/1g60 TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g60 OCA], [https://pdbe.org/1g60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g60 RCSB], [https://www.ebi.ac.uk/pdbsum/1g60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g60 ProSAT], [https://www.topsan.org/Proteins/MCSG/1g60 TOPSAN]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MTM2_MORBO MTM2_MORBO]] This methylase recognizes the double-stranded sequence GAAGA, causes specific methylation on A-5, and protects the DNA from cleavage by the MboII endonuclease. It is not known if the cytosine of the complementary sequence TCTTC is also methylated by this enzyme.
+[[https://www.uniprot.org/uniprot/MTM2_MORBO MTM2_MORBO]] This methylase recognizes the double-stranded sequence GAAGA, causes specific methylation on A-5, and protects the DNA from cleavage by the MboII endonuclease. It is not known if the cytosine of the complementary sequence TCTTC is also methylated by this enzyme.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

1g60

From Proteopedia

Revision as of 11:19, 28 July 2021

Crystal Structure of Methyltransferase MboIIa (Moraxella bovis)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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