1eyn

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[[Image:1eyn.jpg|left|200px]]
[[Image:1eyn.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1eyn |SIZE=350|CAPTION= <scene name='initialview01'>1eyn</scene>, resolution 1.7&Aring;
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The line below this paragraph, containing "STRUCTURE_1eyn", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=2AN:1-ANILINO-8-NAPHTHALENE+SULFONATE'>2AN</scene>, <scene name='pdbligand=ASI:L-ISO-ASPARTATE'>ASI</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1eyn| PDB=1eyn | SCENE= }}
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|RELATEDENTRY=[[1ejc|1EJC]], [[1ejd|1EJD]], [[1dlg|1DLG]], [[1naw|1NAW]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eyn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyn OCA], [http://www.ebi.ac.uk/pdbsum/1eyn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1eyn RCSB]</span>
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}}
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'''STRUCTURE OF MURA LIGANDED WITH THE EXTRINSIC FLUORESCENCE PROBE ANS'''
'''STRUCTURE OF MURA LIGANDED WITH THE EXTRINSIC FLUORESCENCE PROBE ANS'''
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[[Category: Luger, K.]]
[[Category: Luger, K.]]
[[Category: Schonbrunn, E.]]
[[Category: Schonbrunn, E.]]
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[[Category: inside-out alpha-beta barrel]]
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[[Category: Inside-out alpha-beta barrel]]
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[[Category: l-isoaspartate in position 67]]
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[[Category: L-isoaspartate in position 67]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:40:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:12:14 2008''
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Revision as of 12:40, 2 May 2008

Image:1eyn.jpg

Template:STRUCTURE 1eyn

STRUCTURE OF MURA LIGANDED WITH THE EXTRINSIC FLUORESCENCE PROBE ANS


Overview

The extrinsic fluorescence dye 8-anilino-1-naphthalene sulfonate (ANS) is widely used for probing conformational changes in proteins, yet no detailed structure of ANS bound to any protein has been reported so far. ANS has been successfully used to monitor the induced-fit mechanism of MurA [UDPGlcNAc enolpyruvyltransferase (EC )], an essential enzyme for bacterial cell wall biosynthesis. We have solved the crystal structure of the ANS small middle dotMurA complex at 1.7-A resolution. ANS binds at an originally solvent-exposed region near Pro-112 and induces a major restructuring of the loop Pro-112-Pro-121, such that a specific binding site emerges. The fluorescence probe is sandwiched between the strictly conserved residues Arg-91, Pro-112, and Gly-113. Substrate binding to MurA is accompanied by large movements especially of the loop and Arg-91, which explains why ANS is an excellent sensor of conformational changes during catalysis of this pharmaceutically important enzyme.

About this Structure

1EYN is a Single protein structure of sequence from Enterobacter cloacae. Full crystallographic information is available from OCA.

Reference

Structural basis for the interaction of the fluorescence probe 8-anilino-1-naphthalene sulfonate (ANS) with the antibiotic target MurA., Schonbrunn E, Eschenburg S, Luger K, Kabsch W, Amrhein N, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6345-9. PMID:10823915 Page seeded by OCA on Fri May 2 15:40:38 2008

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OCA

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