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1h6s

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Revision as of 11:33, 28 July 2021

Asymmetric conductivity of engineered proteins

Structural highlights

1h6s is a 1 chain structure with sequence from Atcc 33485. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1bh3, 1prn, 2prn, 3prn, 5prn, 6prn, 7prn, 8prn
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PORI_RHOBL] Forms channels that allow the passive diffusion of small hydrophilic solutes up to an exclusion limit of about 0.6 kDa.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Positively charged peptide segments of 16 and 18 residues were inserted at a periplasmic turn of the porin from Rhodobacter blasticus in order to form an electric field-dependent plug. The X-ray diffraction analysis of a mutant confirmed that the structure of the porin had remained intact and that the insert was mobile. Incorporation experiments of single molecules into lipid bilayers showed that the distribution of electric conduction increments depended on the field polarity. The observed distributions are explained if the porin molecules enter the bilayer preferentially with their periplasmic surface first. Furthermore, the conduction of membrane-incorporated porin mutants changed reproducibly on field reversal showing asymmetries of reverse similar 15%, while the wild-type remained constant. This asymmetry is most likely caused by the electric field pressing the charged insert onto the pore eyelet in one field direction and removing it from the eyelet in the other. The results encourage attempts to improve the inserts in order to eventually reach diode characteristics.

Asymmetric conductivity of engineered porins.,Bannwarth M, Schulz GE Protein Eng. 2002 Oct;15(10):799-804. PMID:12468713^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bannwarth M, Schulz GE. Asymmetric conductivity of engineered porins. Protein Eng. 2002 Oct;15(10):799-804. PMID:12468713

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1h6s"

@@ Line 3: / Line 3: @@
 <StructureSection load='1h6s' size='340' side='right'caption='[[1h6s]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1h6s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33485 Atcc 33485]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H6S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H6S FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1h6s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_33485 Atcc 33485]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H6S FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bh3|1bh3]], [[1prn|1prn]], [[2prn|2prn]], [[3prn|3prn]], [[5prn|5prn]], [[6prn|6prn]], [[7prn|7prn]], [[8prn|8prn]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bh3|1bh3]], [[1prn|1prn]], [[2prn|2prn]], [[3prn|3prn]], [[5prn|5prn]], [[6prn|6prn]], [[7prn|7prn]], [[8prn|8prn]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h6s OCA], [http://pdbe.org/1h6s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h6s RCSB], [http://www.ebi.ac.uk/pdbsum/1h6s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1h6s ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h6s OCA], [https://pdbe.org/1h6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h6s RCSB], [https://www.ebi.ac.uk/pdbsum/1h6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h6s ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PORI_RHOBL PORI_RHOBL]] Forms channels that allow the passive diffusion of small hydrophilic solutes up to an exclusion limit of about 0.6 kDa.
+[[https://www.uniprot.org/uniprot/PORI_RHOBL PORI_RHOBL]] Forms channels that allow the passive diffusion of small hydrophilic solutes up to an exclusion limit of about 0.6 kDa.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 30: @@
 ==See Also==
-*[[Porin|Porin]]
+*[[Porin 3D structures|Porin 3D structures]]
 == References ==
 <references/>

1h6s

From Proteopedia

Revision as of 11:33, 28 July 2021

Asymmetric conductivity of engineered proteins

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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