1ezv

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[[Image:1ezv.gif|left|200px]]
[[Image:1ezv.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ezv |SIZE=350|CAPTION= <scene name='initialview01'>1ezv</scene>, resolution 2.3&Aring;
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The line below this paragraph, containing "STRUCTURE_1ezv", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SMA:STIGMATELLIN+A'>SMA</scene>, <scene name='pdbligand=UQ6:5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL'>UQ6</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquinol--cytochrome-c_reductase Ubiquinol--cytochrome-c reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.2.2 1.10.2.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1ezv| PDB=1ezv | SCENE= }}
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|RELATEDENTRY=[[1qcr|1QCR]], [[3bcc|3BCC]], [[1bcc|1BCC]], [[2bcc|2BCC]], [[1be3|1BE3]], [[1bgy|1BGY]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ezv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ezv OCA], [http://www.ebi.ac.uk/pdbsum/1ezv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ezv RCSB]</span>
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}}
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'''STRUCTURE OF THE YEAST CYTOCHROME BC1 COMPLEX CO-CRYSTALLIZED WITH AN ANTIBODY FV-FRAGMENT'''
'''STRUCTURE OF THE YEAST CYTOCHROME BC1 COMPLEX CO-CRYSTALLIZED WITH AN ANTIBODY FV-FRAGMENT'''
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[[Category: Michel, H.]]
[[Category: Michel, H.]]
[[Category: Rossmanith, T.]]
[[Category: Rossmanith, T.]]
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[[Category: antibody fv-fragment]]
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[[Category: Antibody fv-fragment]]
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[[Category: coenzyme q6]]
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[[Category: Coenzyme q6]]
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[[Category: complex iii]]
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[[Category: Complex iii]]
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[[Category: cytochrome bc1 complex]]
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[[Category: Cytochrome bc1 complex]]
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[[Category: electron transfer]]
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[[Category: Electron transfer]]
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[[Category: matrix processing peptidase]]
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[[Category: Matrix processing peptidase]]
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[[Category: mitochondria]]
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[[Category: Mitochondria]]
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[[Category: proton transfer]]
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[[Category: Proton transfer]]
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[[Category: q-cycle]]
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[[Category: Q-cycle]]
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[[Category: qcr]]
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[[Category: Qcr]]
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[[Category: stigmatellin]]
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[[Category: Stigmatellin]]
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[[Category: ubiquinone]]
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[[Category: Ubiquinone]]
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[[Category: yeast]]
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[[Category: Yeast]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:43:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:12:57 2008''
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Revision as of 12:43, 2 May 2008

Image:1ezv.gif

Template:STRUCTURE 1ezv

STRUCTURE OF THE YEAST CYTOCHROME BC1 COMPLEX CO-CRYSTALLIZED WITH AN ANTIBODY FV-FRAGMENT


Overview

BACKGROUND: The cytochrome bc(1) complex is part of the energy conversion machinery of the respiratory and photosynthetic electron transfer chains. This integral membrane protein complex catalyzes electron transfer from ubiquinol to cytochrome c. It couples the electron transfer to the electrogenic translocation of protons across the membrane via a so-called Q cycle mechanism. RESULTS: The cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae was crystallized together with a bound antibody Fv fragment. The structure was determined at 2.3 A resolution using multiple isomorphous replacement, and refined to a crystallographic R factor of 22.2% (R(free) = 25.4%). The complex is present as a homodimer. Each 'monomer' of the refined model includes 2178 amino acid residues of subunits COR1, QCR2, COB, CYT1, RIP1, QCR6, QCR7, QCR8 and QCR9 of the cytochrome bc(1) complex and of the polypeptides V(H) and V(L) of the Fv fragment, the cofactors heme b(H), heme b(L), heme c(1), the [2Fe-2S] cluster and 346 water molecules. The Fv fragment binds to the extrinsic domain of the [2Fe-2S] Rieske protein and is essential for formation of the crystal lattice. CONCLUSIONS: The approach to crystallize membrane proteins as complexes with specific antibody fragments appears to be of general importance. The structure of the yeast cytochrome bc(1) complex reveals in detail the binding sites of the natural substrate coenzyme Q6 and the inhibitor stigmatellin. Buried water molecules close to the binding sites suggest possible pathways for proton uptake and release. A comparison with other cytochrome bc(1) complexes shows features that are specific to yeast.

About this Structure

1EZV is a Protein complex structure of sequences from Mus musculus and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure at 2.3 A resolution of the cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment., Hunte C, Koepke J, Lange C, Rossmanith T, Michel H, Structure. 2000 Jun 15;8(6):669-84. PMID:10873857 Page seeded by OCA on Fri May 2 15:43:13 2008

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