1j3q

From Proteopedia

(Difference between revisions)

Revision as of 11:10, 4 August 2021

Crystal structure of Thermococcus litoralis phosphogrucose isomerase soaked with FeSO4

Structural highlights

1j3q is a 2 chain structure with sequence from 'caldococcus litoralis' z-1301. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1j3p, 1j3r
Activity:	Glucose-6-phosphate isomerase, with EC number 5.3.1.9
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The gene encoding phosphoglucose isomerase was cloned from Thermococcus litoralis, and functionally expressed in Escherichia coli. The purified enzyme, a homodimer of 21.5 kDa subunits, was biochemically characterized. The inhibition constants for four competitive inhibitors were determined. The enzyme contained 1.25 mol Fe and 0.24 mol Zn per dimer. The activity was enhanced by the addition of Fe(2+), but inhibited by Zn(2+) and EDTA. Enzymes with mutations in conserved histidine and glutamate residues in their cupin motifs contained no metals, and showed large decreases in k(cat). The circular dichroism spectra of the mutant enzymes and the wild type enzyme were essentially the same but with slight differences.

Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis.,Jeong JJ, Fushinobu S, Ito S, Jeon BS, Shoun H, Wakagi T FEBS Lett. 2003 Jan 30;535(1-3):200-4. PMID:12560104^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jeong JJ, Fushinobu S, Ito S, Jeon BS, Shoun H, Wakagi T. Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis. FEBS Lett. 2003 Jan 30;535(1-3):200-4. PMID:12560104

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1j3q"

@@ Line 3: / Line 3: @@
 <StructureSection load='1j3q' size='340' side='right'caption='[[1j3q]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1j3q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'caldococcus_litoralis'_z-1301 'caldococcus litoralis' z-1301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J3Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J3Q FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1j3q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/'caldococcus_litoralis'_z-1301 'caldococcus litoralis' z-1301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J3Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J3Q FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1j3p|1j3p]], [[1j3r|1j3r]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1j3p|1j3p]], [[1j3r|1j3r]]</div></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j3q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j3q OCA], [http://pdbe.org/1j3q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1j3q RCSB], [http://www.ebi.ac.uk/pdbsum/1j3q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1j3q ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j3q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j3q OCA], [https://pdbe.org/1j3q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j3q RCSB], [https://www.ebi.ac.uk/pdbsum/1j3q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j3q ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 30: / Line 30: @@
 ==See Also==
-*[[Phosphoglucoisomerase|Phosphoglucoisomerase]]
+*[[Phosphoglucose isomerase 3D structures|Phosphoglucose isomerase 3D structures]]
-*[[Phosphoglucose isomerase|Phosphoglucose isomerase]]
 == References ==
 <references/>

1j3q

From Proteopedia

Revision as of 11:10, 4 August 2021

Crystal structure of Thermococcus litoralis phosphogrucose isomerase soaked with FeSO4

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

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