1f08
From Proteopedia
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'''CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS''' | '''CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS''' | ||
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[[Category: Stenlund, A.]] | [[Category: Stenlund, A.]] | ||
[[Category: Vaughn, D E.]] | [[Category: Vaughn, D E.]] | ||
| - | [[Category: | + | [[Category: Dna-binding domain]] |
| - | [[Category: | + | [[Category: Helicase]] |
| - | [[Category: | + | [[Category: Initiator protein]] |
| - | [[Category: | + | [[Category: Papillomavirus]] |
| - | [[Category: | + | [[Category: Replication]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:44:14 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:44, 2 May 2008
CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS
Overview
Papillomaviral infection causes both benign and malignant lesions and is a necessary cause of cervical carcinoma. Replication of this virus requires the replication initiation proteins E1 and E2, which bind cooperatively at the origin of replication (ori) as an (E1)2-(E2)2-DNA complex. This is a precursor to larger E1 complexes that distort and unwind the ori. We present the crystal structure of the E1 DNA binding domain refined to 1.9 A resolution. Residues critical for DNA binding are located on an extended loop and an alpha helix. We identify the E1 dimerization surface by selective mutations at an E1/E1 interface observed in the crystal and propose a model for the (E1)2-DNA complex. These and other observations suggest how the E1 DNA binding domain orchestrates assembly of the hexameric helicase on the ori.
About this Structure
1F08 is a Single protein structure of sequence from Bovine papillomavirus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the DNA binding domain of the replication initiation protein E1 from papillomavirus., Enemark EJ, Chen G, Vaughn DE, Stenlund A, Joshua-Tor L, Mol Cell. 2000 Jul;6(1):149-58. PMID:10949036 Page seeded by OCA on Fri May 2 15:44:14 2008
