7bk8
From Proteopedia
(Difference between revisions)
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==X-ray crystal structure of Pseudomonas aeruginosa MagC== | ==X-ray crystal structure of Pseudomonas aeruginosa MagC== | ||
| - | <StructureSection load='7bk8' size='340' side='right'caption='[[7bk8]]' scene=''> | + | <StructureSection load='7bk8' size='340' side='right'caption='[[7bk8]], [[Resolution|resolution]] 1.74Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BK8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7bk8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BK8 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bk8 OCA], [https://pdbe.org/7bk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bk8 RCSB], [https://www.ebi.ac.uk/pdbsum/7bk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bk8 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA4490 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bk8 OCA], [https://pdbe.org/7bk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bk8 RCSB], [https://www.ebi.ac.uk/pdbsum/7bk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bk8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial alpha-2 macroglobulins (A2Ms) structurally resemble the large spectrum protease inhibitors of the eukaryotic immune system. In Pseudomonas aeruginosa, MagD acts as an A2M and is expressed within a six-gene operon encoding the MagA-F proteins. In this work, we employ isothermal calorimetry (ITC), analytical ultracentrifugation (AUC), and X-ray crystallography to investigate the function of MagC and show that MagC associates with the macroglobulin complex and with the peptidoglycan (PG). However, the catalytic residues of MagC display an inactive conformation that could suggest that it binds to PG but does not degrade it. We hypothesize that MagC could serve as an anchor between the MagD macroglobulin and the PG and could provide stabilization and/or regulation for the entire complex. | ||
| + | |||
| + | MagC is a NplC/P60-like member of the alpha-2-macroglobulin Mag complex of Pseudomonas aeruginosa that interacts with peptidoglycan.,Zouhir S, Contreras-Martel C, Maragno Trindade D, Attree I, Dessen A, Macheboeuf P FEBS Lett. 2021 Jun 11. doi: 10.1002/1873-3468.14148. PMID:34115884<ref>PMID:34115884</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bk8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Attree I]] | + | [[Category: Pseae]] |
| - | [[Category: Contreras-Martel C]] | + | [[Category: Attree, I]] |
| - | [[Category: Dessen A]] | + | [[Category: Contreras-Martel, C]] |
| - | [[Category: Macheboeuf P]] | + | [[Category: Dessen, A]] |
| - | [[Category: | + | [[Category: Macheboeuf, P]] |
| - | [[Category: Zouhir S]] | + | [[Category: Trindade, D Maragno]] |
| + | [[Category: Zouhir, S]] | ||
| + | [[Category: Alpha-2-macroglobulin]] | ||
| + | [[Category: Endopeptidase]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Magc]] | ||
| + | [[Category: Nlpc/p60]] | ||
| + | [[Category: Peptidoglycan]] | ||
Revision as of 06:15, 18 August 2021
X-ray crystal structure of Pseudomonas aeruginosa MagC
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