1f4l

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[[Image:1f4l.jpg|left|200px]]
[[Image:1f4l.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1f4l |SIZE=350|CAPTION= <scene name='initialview01'>1f4l</scene>, resolution 1.85&Aring;
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The line below this paragraph, containing "STRUCTURE_1f4l", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine--tRNA_ligase Methionine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.10 6.1.1.10] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= SYNTHETIC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1f4l| PDB=1f4l | SCENE= }}
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|RELATEDENTRY=[[1qqt|1QQT]], [[1a8h|1A8H]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f4l OCA], [http://www.ebi.ac.uk/pdbsum/1f4l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f4l RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF THE E.COLI METHIONYL-TRNA SYNTHETASE COMPLEXED WITH METHIONINE'''
'''CRYSTAL STRUCTURE OF THE E.COLI METHIONYL-TRNA SYNTHETASE COMPLEXED WITH METHIONINE'''
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[[Category: Serre, L.]]
[[Category: Serre, L.]]
[[Category: Verdon, G.]]
[[Category: Verdon, G.]]
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[[Category: zelwer, C.]]
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[[Category: Zelwer, C.]]
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[[Category: amino acid]]
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[[Category: Amino acid]]
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[[Category: rossman fold]]
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[[Category: Rossman fold]]
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[[Category: trna]]
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[[Category: Trna]]
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[[Category: zinc domain]]
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[[Category: Zinc domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:53:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:15:44 2008''
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Revision as of 12:53, 2 May 2008

Image:1f4l.jpg

Template:STRUCTURE 1f4l

CRYSTAL STRUCTURE OF THE E.COLI METHIONYL-TRNA SYNTHETASE COMPLEXED WITH METHIONINE


Overview

Amino acid selection by aminoacyl-tRNA synthetases requires efficient mechanisms to avoid incorrect charging of the cognate tRNAs. A proofreading mechanism prevents Escherichia coli methionyl-tRNA synthetase (EcMet-RS) from activating in vivo L-homocysteine, a natural competitor of L-methionine recognised by the enzyme. The crystal structure of the complex between EcMet-RS and L-methionine solved at 1.8 A resolution exhibits some conspicuous differences with the recently published free enzyme structure. Thus, the methionine delta-sulphur atom replaces a water molecule H-bonded to Leu13N and Tyr260O(eta) in the free enzyme. Rearrangements of aromatic residues enable the protein to form a hydrophobic pocket around the ligand side-chain. The subsequent formation of an extended water molecule network contributes to relative displacements, up to 3 A, of several domains of the protein. The structure of this complex supports a plausible mechanism for the selection of L-methionine versus L-homocysteine and suggests the possibility of information transfer between the different functional domains of the enzyme.

About this Structure

1F4L is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

How methionyl-tRNA synthetase creates its amino acid recognition pocket upon L-methionine binding., Serre L, Verdon G, Choinowski T, Hervouet N, Risler JL, Zelwer C, J Mol Biol. 2001 Mar 2;306(4):863-76. PMID:11243794 Page seeded by OCA on Fri May 2 15:53:32 2008

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