3ket

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==Crystal structure of a Rex-family transcriptional regulatory protein from Streptococcus agalactiae bound to a palindromic operator==
==Crystal structure of a Rex-family transcriptional regulatory protein from Streptococcus agalactiae bound to a palindromic operator==
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<StructureSection load='3ket' size='340' side='right' caption='[[3ket]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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<StructureSection load='3ket' size='340' side='right'caption='[[3ket]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ket]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_(serotype_iii) Streptococcus agalactiae (serotype iii)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KET FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ket]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_(serotype_iii) Streptococcus agalactiae (serotype iii)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KET FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3keq|3keq]], [[3keo|3keo]], [[2vt3|2vt3]], [[2dt5|2dt5]]</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3keq|3keq]], [[3keo|3keo]], [[2vt3|2vt3]], [[2dt5|2dt5]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gbs1167, rex ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216495 Streptococcus agalactiae (serotype III)])</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gbs1167, rex ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216495 Streptococcus agalactiae (serotype III)])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ket FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ket OCA], [http://pdbe.org/3ket PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ket RCSB], [http://www.ebi.ac.uk/pdbsum/3ket PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ket ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ket FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ket OCA], [https://pdbe.org/3ket PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ket RCSB], [https://www.ebi.ac.uk/pdbsum/3ket PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ket ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/REX_STRA3 REX_STRA3]] Modulates transcription in response to changes in cellular NADH/NAD(+) redox state (By similarity).
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[[https://www.uniprot.org/uniprot/REX_STRA3 REX_STRA3]] Modulates transcription in response to changes in cellular NADH/NAD(+) redox state (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In many Gram-positive bacteria, the redox-sensing transcriptional repressor Rex controls central carbon and energy metabolism by sensing the intra cellular balance between the reduced and oxidized forms of nicotinamide adenine dinucleotide; the NADH/NAD+ ratio. Here, we report high-resolution crystal structures and characterization of a Rex ortholog (Gbs1167) in the opportunistic pathogen, Streptococcus agalactiae, also known as group B streptococcus (GBS). We present structures of Rex bound to NAD+ and to a DNA operator which are the first structures of a Rex-family member from a pathogenic bacterium. The structures reveal the molecular basis of DNA binding and the conformation alterations between the free NAD+ complex and DNA-bound form of Rex. Transcriptomic analysis revealed that GBS Rex controls not only central metabolism, but also expression of the monocistronic rex gene as well as virulence gene expression. Rex enhances GBS virulence after disseminated infection in mice. Mechanistically, NAD+ stabilizes Rex as a repressor in the absence of NADH. However, GBS Rex is unique compared to Rex regulators previously characterized because of its sensing mechanism: we show that it primarily responds to NAD+ levels (or growth rate) rather than to the NADH/NAD+ ratio. These results indicate that Rex plays a key role in GBS pathogenicity by modulating virulence factor gene expression and carbon metabolism to harvest nutrients from the host.
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NAD+ pool depletion as a signal for the Rex regulon involved in Streptococcus agalactiae virulence.,Franza T, Rogstam A, Thiyagarajan S, Sullivan MJ, Derre-Bobillot A, Bauer MC, Goh KGK, Da Cunha V, Glaser P, Logan DT, Ulett GC, von Wachenfeldt C, Gaudu P PLoS Pathog. 2021 Aug 9;17(8):e1009791. doi: 10.1371/journal.ppat.1009791., eCollection 2021 Aug. PMID:34370789<ref>PMID:34370789</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ket" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Logan, D]]
[[Category: Logan, D]]
[[Category: Thiyagarajan, S]]
[[Category: Thiyagarajan, S]]

Revision as of 09:00, 8 September 2021

Crystal structure of a Rex-family transcriptional regulatory protein from Streptococcus agalactiae bound to a palindromic operator

PDB ID 3ket

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