1f82

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1f82.jpg|left|200px]]
[[Image:1f82.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1f82 |SIZE=350|CAPTION= <scene name='initialview01'>1f82</scene>, resolution 2.20&Aring;
+
The line below this paragraph, containing "STRUCTURE_1f82", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1f82| PDB=1f82 | SCENE= }}
-
|RELATEDENTRY=[[3bta|3BTA]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f82 OCA], [http://www.ebi.ac.uk/pdbsum/1f82 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f82 RCSB]</span>
+
-
}}
+
'''BOTULINUM NEUROTOXIN TYPE B CATALYTIC DOMAIN'''
'''BOTULINUM NEUROTOXIN TYPE B CATALYTIC DOMAIN'''
Line 28: Line 25:
[[Category: Hanson, M A.]]
[[Category: Hanson, M A.]]
[[Category: Stevens, R C.]]
[[Category: Stevens, R C.]]
-
[[Category: botulinum neurotoxin]]
+
[[Category: Botulinum neurotoxin]]
-
[[Category: zinc dependent protease]]
+
[[Category: Zinc dependent protease]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:00:52 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:17:46 2008''
+

Revision as of 13:00, 2 May 2008

Image:1f82.jpg

Template:STRUCTURE 1f82

BOTULINUM NEUROTOXIN TYPE B CATALYTIC DOMAIN


Overview

Botulinum neurotoxin serotype B is a zinc protease that disrupts neurotransmitter release by cleaving synaptobrevin-II (Sb2), one of three SNARE proteins involved in neuronal synaptic vesicle fusion. The three-dimensional crystal structure of the apo botulinum neurotoxin serotype B catalytic domain (BoNT/B-LC) has been determined to 2.2 A resolution, and the complex of cleaved Sb2 with the catalytic domain (Sb2-BoNT/B-LC) has been determined to 2.0 A resolution. A comparison of the holotoxin catalytic domain and the isolated BoNT/B-LC structure shows a rearrangement of three active site loops. This rearrangement exposes the BoNT/B active site. The Sb2-BoNT/B-LC structure illustrates two distinct binding regions, which explains the specificity of each botulinum neurotoxin for its synaptic vesicle protein. This observation provides an explanation for the proposed cooperativity between binding of full-length substrate and catalysis and suggest a mechanism of synaptobrevin proteolysis employed by the clostridial neurotoxins.

About this Structure

1F82 is a Single protein structure of sequence from Clostridium botulinum. Full crystallographic information is available from OCA.

Reference

Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin type B at 2.0 A resolution., Hanson MA, Stevens RC, Nat Struct Biol. 2000 Aug;7(8):687-92. PMID:10932255 Page seeded by OCA on Fri May 2 16:00:52 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1f82"
Personal tools