==Crystal structure of the T(-30) Adenovirus major late promoter TATA box variant bound to wild-type TBP (Arabidopsis thaliana TBP isoform 2). TATA element recognition by the TATA box-binding protein has been conserved throughout evolution.==

<StructureSection load='1qna' size='340' side='right'caption='[[1qna]], [[Resolution|resolution]] 1.80&Aring;' scene=''>

<table><tr><td colspan='2'>[[1qna]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QNA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QNA FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1qn3|1qn3]], [[1qn4|1qn4]], [[1qn5|1qn5]], [[1qn6|1qn6]], [[1qn7|1qn7]], [[1qn8|1qn8]], [[1qn9|1qn9]], [[1qnb|1qnb]], [[1qnc|1qnc]], [[1qne|1qne]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qna OCA], [http://pdbe.org/1qna PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qna RCSB], [http://www.ebi.ac.uk/pdbsum/1qna PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qna ProSAT]</span></td></tr>

== Function ==

[[http://www.uniprot.org/uniprot/TBP1_ARATH TBP1_ARATH]] General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

Cocrystal structures of wild-type TATA box-binding protein (TBP) recognizing 10 naturally occurring TATA elements have been determined at 2.3-1.8 A resolution, and compared with our 1.9 A resolution structure of TBP bound to the Adenovirus major late promoter (AdMLP) TATA box (5'-TATAAAAG-3'). Minor-groove recognition by the saddle-shaped protein induces the same conformational change in each of these oligonucleotides, despite variations in promoter sequence that reduce the efficiency of transcription initiation. Three molecular mechanisms explain assembly of diverse TBP-TATA element complexes. (1) T --&gt; A and A --&gt; T transversions leave the minor-groove face unchanged, permitting formation of TBP-DNA complexes on many A/T-rich core promoter sequences. (2) Cavities in the interface between TBP and the minor-groove face of the AdMLP TATA box accommodate the exocyclic NH(2) groups of G in a TACA box and in a TATAAG box. (3) Formation of a C:G Hoogsteen basepair in a TATAAAC box eliminates steric clashes that would be produced by the Watson-Crick base pair. We conclude that the structure of the TBP-TATA box complex found at the heart of the polymerase II (pol II) transcription machinery has remained constant over the course of evolution, despite variations in TBP and its DNA targets.

TATA element recognition by the TATA box-binding protein has been conserved throughout evolution.,Patikoglou GA, Kim JL, Sun L, Yang SH, Kodadek T, Burley SK Genes Dev. 1999 Dec 15;13(24):3217-30. PMID:10617571<ref>PMID:10617571</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 1qna" style="background-color:#fffaf0;"></div>

==See Also==

*[[TATA-Binding Protein|TATA-Binding Protein]]

*[[Transcription initiation factor|Transcription initiation factor]]

== References ==

<references/>

[[Category: Arath]]

[[Category: Burley, S K]]

[[Category: Tbp-tata element complex]]