1f8m

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1f8m.gif|left|200px]]
[[Image:1f8m.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1f8m |SIZE=350|CAPTION= <scene name='initialview01'>1f8m</scene>, resolution 1.80&Aring;
+
The line below this paragraph, containing "STRUCTURE_1f8m", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Isocitrate_lyase Isocitrate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.1 4.1.3.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG2224 AceA]</span>
+
{{STRUCTURE_1f8m| PDB=1f8m | SCENE= }}
-
|RELATEDENTRY=[[1f61|1F61]], [[1f8i|1F8I]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f8m OCA], [http://www.ebi.ac.uk/pdbsum/1f8m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f8m RCSB]</span>
+
-
}}
+
'''CRYSTAL STRUCTURE OF 3-BROMOPYRUVATE MODIFIED ISOCITRATE LYASE (ICL) FROM MYCOBACTERIUM TUBERCULOSIS'''
'''CRYSTAL STRUCTURE OF 3-BROMOPYRUVATE MODIFIED ISOCITRATE LYASE (ICL) FROM MYCOBACTERIUM TUBERCULOSIS'''
Line 34: Line 31:
[[Category: Sharma, V.]]
[[Category: Sharma, V.]]
[[Category: TBSGC, TB Structural Genomics Consortium.]]
[[Category: TBSGC, TB Structural Genomics Consortium.]]
-
[[Category: alpha-beta barrel]]
+
[[Category: Alpha-beta barrel]]
-
[[Category: bromopyuvate modification]]
+
[[Category: Bromopyuvate modification]]
-
[[Category: closed conformation]]
+
[[Category: Closed conformation]]
-
[[Category: helix-swapping]]
+
[[Category: Helix-swapping]]
-
[[Category: protein structure initiative]]
+
[[Category: Protein structure initiative]]
-
[[Category: psi]]
+
[[Category: Psi]]
-
[[Category: structural genomic]]
+
[[Category: Structural genomic]]
-
[[Category: tb structural genomics consortium]]
+
[[Category: Tb structural genomics consortium]]
-
[[Category: tbsgc]]
+
[[Category: Tbsgc]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:01:53 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:18:06 2008''
+

Revision as of 13:01, 2 May 2008

Image:1f8m.gif

Template:STRUCTURE 1f8m

CRYSTAL STRUCTURE OF 3-BROMOPYRUVATE MODIFIED ISOCITRATE LYASE (ICL) FROM MYCOBACTERIUM TUBERCULOSIS


Overview

Isocitrate lyase (ICL) plays a pivotal role in the persistence of Mycobacterium tuberculosis in mice by sustaining intracellular infection in inflammatory macrophages. The enzyme allows net carbon gain by diverting acetyl-CoA from beta-oxidation of fatty acids into the glyoxylate shunt pathway. Given its potential as a drug target against persistent infections, we solved its structure without ligand and in complex with two inhibitors. Covalent modification of an active site residue, Cys 191, by the inhibitor 3-bromopyruvate traps the enzyme in a catalytic conformation with the active site completely inaccessible to solvent. The structure of a C191S mutant of the enzyme with the inhibitor 3-nitropropionate provides further insight into the reaction mechanism.

About this Structure

1F8M is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Structure of isocitrate lyase, a persistence factor of Mycobacterium tuberculosis., Sharma V, Sharma S, Hoener zu Bentrup K, McKinney JD, Russell DG, Jacobs WR Jr, Sacchettini JC, Nat Struct Biol. 2000 Aug;7(8):663-8. PMID:10932251 Page seeded by OCA on Fri May 2 16:01:53 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1f8m"
Personal tools