6yvc
From Proteopedia
(Difference between revisions)
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==Crystal structure of the small alarmone hydrolase (SAH) of Pseudomonas aeruginosa== | ==Crystal structure of the small alarmone hydrolase (SAH) of Pseudomonas aeruginosa== | ||
| - | <StructureSection load='6yvc' size='340' side='right'caption='[[6yvc]]' scene=''> | + | <StructureSection load='6yvc' size='340' side='right'caption='[[6yvc]], [[Resolution|resolution]] 1.85Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YVC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6yvc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YVC FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yvc OCA], [https://pdbe.org/6yvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yvc RCSB], [https://www.ebi.ac.uk/pdbsum/6yvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yvc ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">C0044_03060, CGU42_31275, DT376_11420, DY930_06745, DZ962_15010, ECC04_012485, F3H14_24895, IPC116_15200, IPC1481_11715, IPC1509_07345, IPC36_18005, PAMH19_0488 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yvc OCA], [https://pdbe.org/6yvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yvc RCSB], [https://www.ebi.ac.uk/pdbsum/6yvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yvc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The guanosine nucleotide-based second messengers ppGpp and pppGpp (collectively: (p)ppGpp) enable adaptation of microorganisms to environmental changes and stress conditions. In contrast, the closely related adenosine nucleotides (p)ppApp are involved in type VI secretion system (T6SS)-mediated killing during bacterial competition. Long RelA-SpoT Homolog (RSH) enzymes regulate synthesis and degradation of (p)ppGpp (and potentially also (p)ppApp) through their synthetase and hydrolase domains, respectively. Small alarmone hydrolases (SAH) that consist of only a hydrolase domain are found in a variety of bacterial species, including the opportunistic human pathogen Pseudomonas aeruginosa. Here, we present the structure and mechanism of P. aeruginosa SAH showing that the enzyme promiscuously hydrolyses (p)ppGpp and (p)ppApp in a strictly manganese-dependent manner. While being dispensable for P. aeruginosa growth or swimming, swarming, and twitching motilities, its enzymatic activity is required for biofilm formation. Moreover, (p)ppApp-degradation by SAH provides protection against the T6SS (p)ppApp synthetase effector Tas1, suggesting that SAH enzymes can also serve as defense proteins during interbacterial competition. | ||
| + | |||
| + | Dual role of a (p)ppGpp- and (p)ppApp-degrading enzyme in biofilm formation and interbacterial antagonism.,Steinchen W, Ahmad S, Valentini M, Eilers K, Majkini M, Altegoer F, Lechner M, Filloux A, Whitney JC, Bange G Mol Microbiol. 2021 Jun;115(6):1339-1356. doi: 10.1111/mmi.14684. Epub 2021 Jan, 25. PMID:33448498<ref>PMID:33448498</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6yvc" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Altegoer F]] | + | [[Category: Altegoer, F]] |
| - | [[Category: Bange G]] | + | [[Category: Bange, G]] |
| + | [[Category: Alarmone]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Pppgpp]] | ||
| + | [[Category: Pseudomonas aeruginosa]] | ||
Revision as of 12:46, 13 October 2021
Crystal structure of the small alarmone hydrolase (SAH) of Pseudomonas aeruginosa
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