1ffq

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[[Image:1ffq.gif|left|200px]]
[[Image:1ffq.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ffq |SIZE=350|CAPTION= <scene name='initialview01'>1ffq</scene>, resolution 1.9&Aring;
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The line below this paragraph, containing "STRUCTURE_1ffq", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=AMI:ALLOSAMIZOLINE'>AMI</scene>, <scene name='pdbligand=NAA:N-ACETYL-D-ALLOSAMINE'>NAA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1ffq| PDB=1ffq | SCENE= }}
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|RELATEDENTRY=[[1edq|1EDQ]], [[1ehn|1EHN]], [[1eib|1EIB]], [[1ffr|1FFR]], [[1ctn|1CTN]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ffq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ffq OCA], [http://www.ebi.ac.uk/pdbsum/1ffq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ffq RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF CHITINASE A COMPLEXED WITH ALLOSAMIDIN'''
'''CRYSTAL STRUCTURE OF CHITINASE A COMPLEXED WITH ALLOSAMIDIN'''
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[[Category: Tavlas, G.]]
[[Category: Tavlas, G.]]
[[Category: Vorgias, C E.]]
[[Category: Vorgias, C E.]]
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[[Category: enzyme-inhibitor complex]]
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[[Category: Enzyme-inhibitor complex]]
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[[Category: glycosyl hydrolase]]
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[[Category: Glycosyl hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:16:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:21:58 2008''
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Revision as of 13:16, 2 May 2008

Image:1ffq.gif

Template:STRUCTURE 1ffq

CRYSTAL STRUCTURE OF CHITINASE A COMPLEXED WITH ALLOSAMIDIN


Overview

The purification scheme of chitinase A (ChiA) from S. marcescens has been extensively revised. The pure enzyme crystallizes readily under new crystallization conditions. The ChiA crystal structure has been refined to 1.55 A resolution and the crystal structure of ChiA co-crystallized with the inhibitor allosamidin has been refined to 1.9 A resolution. Allosamidin is located in the deep active-site tunnel of ChiA and interacts with three important residues: Glu315, the proton donor of the catalysis, Asp313, which adopts two conformations in the native structure but is oriented towards Glu315 in the inhibitor complex, and Tyr390, which lies opposite Glu315 in the active-site tunnel.

About this Structure

1FFQ is a Single protein structure of sequence from Serratia marcescens. Full crystallographic information is available from OCA.

Reference

De novo purification scheme and crystallization conditions yield high-resolution structures of chitinase A and its complex with the inhibitor allosamidin., Papanikolau Y, Tavlas G, Vorgias CE, Petratos K, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):400-3. Epub 2003, Jan 23. PMID:12554965 Page seeded by OCA on Fri May 2 16:16:12 2008

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