1fj7

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[[Image:1fj7.jpg|left|200px]]
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{{Structure
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fj7 OCA], [http://www.ebi.ac.uk/pdbsum/1fj7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fj7 RCSB]</span>
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'''SOLUTION STRUCTURE OF NUCLEOLIN RBD1'''
'''SOLUTION STRUCTURE OF NUCLEOLIN RBD1'''
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[[Category: Feigon, J.]]
[[Category: Feigon, J.]]
[[Category: Gilbert, D E.]]
[[Category: Gilbert, D E.]]
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[[Category: nucleolus]]
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[[Category: Nucleolus]]
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[[Category: rbd]]
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[[Category: Rbd]]
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[[Category: rna binding domain]]
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[[Category: Rna binding domain]]
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[[Category: rnp]]
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[[Category: Rnp]]
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[[Category: rrm]]
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[[Category: Rrm]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:23:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:23:56 2008''
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Revision as of 13:23, 2 May 2008

Image:1fj7.jpg

Template:STRUCTURE 1fj7

SOLUTION STRUCTURE OF NUCLEOLIN RBD1


Overview

Nucleolin is an abundant 70 kDa nucleolar protein involved in many aspects of ribosomal RNA biogenesis. The central region of nucleolin contains four tandem consensus RNA-binding domains (RBD). The two most N-terminal domains (RBD12) bind with nanomolar affinity to an RNA stem-loop containing the consensus sequence UCCCGA in the loop. We have determined the solution structure of nucleolin RBD12 in its free form and have studied its interaction with a 22 nt RNA stem-loop using multidimensional NMR spectroscopy. The two RBDs adopt the expected beta alpha beta beta alpha beta fold, but the position of the beta 2 strand in both domains differs from what was predicted from sequence alignments. RBD1 and RBD2 are significantly different from each others and this is likely important in their sequence specific recognition of the RNA. RBD1 has a longer alpha-helix 1 and a shorter beta 2-beta 3 loop than RBD2, and differs from most other RBDs in these respects. The two RBDs are separated by a 12 amino acid flexible linker and do not interact with one another in the free protein. This linker becomes ordered when RBD12 binds to the RNA. Analysis of the observed NOEs between the protein and the RNA indicates that both RBDs interact with the RNA loop via their beta-sheet. Each domain binds residues on one side of the loop; specifically, RBD2 contacts the 5' side and RBD1 contacts the 3'.

About this Structure

1FJ7 is a Single protein structure of sequence from Mesocricetus auratus. Full crystallographic information is available from OCA.

Reference

Solution structure of the two N-terminal RNA-binding domains of nucleolin and NMR study of the interaction with its RNA target., Allain FH, Gilbert DE, Bouvet P, Feigon J, J Mol Biol. 2000 Oct 20;303(2):227-41. PMID:11023788 Page seeded by OCA on Fri May 2 16:23:25 2008

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