1flo

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[[Image:1flo.gif|left|200px]]
[[Image:1flo.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1flo |SIZE=350|CAPTION= <scene name='initialview01'>1flo</scene>, resolution 2.65&Aring;
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The line below this paragraph, containing "STRUCTURE_1flo", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=CTA:Active+Catalytic+Site+Of+Monomer+A.+Y343+Is+Donated+By+M+...'>CTA</scene>, <scene name='pdbsite=CTB:Active+Catalytic+Site+Of+Monomer+B.+Y343+Is+Donated+By+M+...'>CTB</scene>, <scene name='pdbsite=CTC:Inactive+Catalytic+Site+Of+Monomer+C.+Y343+Is+Donated+By+...'>CTC</scene>, <scene name='pdbsite=CTD:Inactive+Catalytic+Site+Of+Monomer+D.+Y343+Is+Donated+By+...'>CTD</scene> and <scene name='pdbsite=PHO:Scissle+Phosphates'>PHO</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=PHS:PHOSPHONIC+ACID'>PHS</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= FLP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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-->
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|DOMAIN=
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{{STRUCTURE_1flo| PDB=1flo | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1flo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1flo OCA], [http://www.ebi.ac.uk/pdbsum/1flo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1flo RCSB]</span>
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}}
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'''FLP RECOMBINASE-HOLLIDAY JUNCTION COMPLEX I'''
'''FLP RECOMBINASE-HOLLIDAY JUNCTION COMPLEX I'''
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[[Category: Narendra, U.]]
[[Category: Narendra, U.]]
[[Category: Rice, P A.]]
[[Category: Rice, P A.]]
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[[Category: domain-swapping]]
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[[Category: Domain-swapping]]
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[[Category: holliday-junction]]
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[[Category: Holliday-junction]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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[[Category: tyrosine recombinase]]
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[[Category: Tyrosine recombinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:28:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:25:19 2008''
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Revision as of 13:28, 2 May 2008

Image:1flo.gif

Template:STRUCTURE 1flo

FLP RECOMBINASE-HOLLIDAY JUNCTION COMPLEX I


Overview

The crystal structure of a Flp recombinase tetramer bound to a Holliday junction intermediate has been determined at 2.65 A resolution. Only one of Flp's two domains, containing the active site, is structurally related to other lambda integrase family site-specific recombinases, such as Cre. The Flp active site differs, however, in that the helix containing the nucleophilic tyrosine is domain swapped, such that it cuts its DNA target in trans. The Flp tetramer displays pseudo four-fold symmetry matching that of the square planar Holliday junction substrate. This tetramer is stabilized by additional novel trans interactions among monomers. The structure illustrates how mechanistic unity is maintained on a chemical level while allowing for substantial variation on the structural level within a family of enzymes.

About this Structure

1FLO is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Crystal structure of a Flp recombinase-Holliday junction complex: assembly of an active oligomer by helix swapping., Chen Y, Narendra U, Iype LE, Cox MM, Rice PA, Mol Cell. 2000 Oct;6(4):885-97. PMID:11090626 Page seeded by OCA on Fri May 2 16:28:32 2008

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OCA

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