7mp0
From Proteopedia
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==CRYSTAL STRUCTURE OF NATIVE BOVINE ARRESTIN 1 IN COMPLEX WITH 1D-MYO-INOSITOL 5-DIPHOSPHATE PENTAKISPHOSPHATE (5-PP IP5)== | ==CRYSTAL STRUCTURE OF NATIVE BOVINE ARRESTIN 1 IN COMPLEX WITH 1D-MYO-INOSITOL 5-DIPHOSPHATE PENTAKISPHOSPHATE (5-PP IP5)== | ||
| - | <StructureSection load='7mp0' size='340' side='right'caption='[[7mp0]]' scene=''> | + | <StructureSection load='7mp0' size='340' side='right'caption='[[7mp0]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MP0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MP0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7mp0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MP0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MP0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mp0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mp0 OCA], [https://pdbe.org/7mp0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mp0 RCSB], [https://www.ebi.ac.uk/pdbsum/7mp0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mp0 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ETX:2-ETHOXYETHANOL'>ETX</scene>, <scene name='pdbligand=I7P:(1R,2R,3S,4S,5R,6S)-2,3,4,5,6-PENTAKIS(PHOSPHONOOXY)CYCLOHEXYL+TRIHYDROGEN+DIPHOSPHATE'>I7P</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mp0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mp0 OCA], [https://pdbe.org/7mp0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mp0 RCSB], [https://www.ebi.ac.uk/pdbsum/7mp0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mp0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Disease == | ||
| + | [[https://www.uniprot.org/uniprot/ARRS_BOVIN ARRS_BOVIN]] Note=S-antigen induces autoimmune uveitis. | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/ARRS_BOVIN ARRS_BOVIN]] Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated-phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase. Isoform B plays a role in the phototransduction cascade. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Visual arrestin (Arr1) terminates rhodopsin signaling by blocking its interaction with transducin. To do this, Arr1 translocates from the inner to the outer segment of photoreceptors upon light stimulation. Mounting evidence indicates that inositol phosphates (InsPs) affect Arr1 activity, but the Arr1-InsP molecular interaction remains poorly defined. We report the structure of bovine Arr1 in a ligand-free state featuring a near-complete model of the previously unresolved C-tail, which plays a crucial role in regulating Arr1 activity. InsPs bind to the N-domain basic patch thus displacing the C-tail, suggesting that they prime Arr1 for interaction with rhodopsin and help direct Arr1 translocation. These structures exhibit intact polar cores, suggesting that C-tail removal by InsP binding is insufficient to activate Arr1. These results show how Arr1 activity can be controlled by endogenous InsPs in molecular detail. | ||
| + | |||
| + | Structural evidence for visual arrestin priming via complexation of phosphoinositols.,Sander CL, Luu J, Kim K, Furkert D, Jang K, Reichenwallner J, Kang M, Lee HJ, Eger BT, Choe HW, Fiedler D, Ernst OP, Kim YJ, Palczewski K, Kiser PD Structure. 2021 Oct 21. pii: S0969-2126(21)00370-1. doi:, 10.1016/j.str.2021.10.002. PMID:34678158<ref>PMID:34678158</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7mp0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bos taurus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Kiser | + | [[Category: Kiser, P D]] |
| - | [[Category: Palczewski K]] | + | [[Category: Palczewski, K]] |
| - | [[Category: Sander | + | [[Category: Sander, C L]] |
| + | [[Category: 5pp-ip5]] | ||
| + | [[Category: Gpcr]] | ||
| + | [[Category: Inositol]] | ||
| + | [[Category: Ip7]] | ||
| + | [[Category: Phototransduction]] | ||
| + | [[Category: Protein binding]] | ||
| + | [[Category: Rhodopsin]] | ||
Revision as of 15:59, 3 November 2021
CRYSTAL STRUCTURE OF NATIVE BOVINE ARRESTIN 1 IN COMPLEX WITH 1D-MYO-INOSITOL 5-DIPHOSPHATE PENTAKISPHOSPHATE (5-PP IP5)
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Categories: Bos taurus | Large Structures | Kiser, P D | Palczewski, K | Sander, C L | 5pp-ip5 | Gpcr | Inositol | Ip7 | Phototransduction | Protein binding | Rhodopsin
