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[[Image:1fml.gif|left|200px]]
[[Image:1fml.gif|left|200px]]
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{{Structure
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|PDB= 1fml |SIZE=350|CAPTION= <scene name='initialview01'>1fml</scene>, resolution 2.75&Aring;
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The line below this paragraph, containing "STRUCTURE_1fml", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=A3P:ADENOSINE-3&#39;-5&#39;-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=RTL:RETINOL'>RTL</scene>
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{{STRUCTURE_1fml| PDB=1fml | SCENE= }}
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|RELATEDENTRY=[[1fmj|1FMJ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fml OCA], [http://www.ebi.ac.uk/pdbsum/1fml PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fml RCSB]</span>
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'''CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP'''
'''CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP'''
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[[Category: Newcomer, M E.]]
[[Category: Newcomer, M E.]]
[[Category: Pakhomova, S.]]
[[Category: Pakhomova, S.]]
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[[Category: adenosine 3',5'-diphosphate]]
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[[Category: Adenosine 3',5'-diphosphate]]
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[[Category: dehydratase]]
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[[Category: Dehydratase]]
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[[Category: retinol]]
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[[Category: Retinol]]
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[[Category: sulfotransferase]]
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[[Category: Sulfotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:30:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:25:56 2008''
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Revision as of 13:30, 2 May 2008

Template:STRUCTURE 1fml

CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP


Overview

We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.

About this Structure

1FML is a Single protein structure of sequence from Spodoptera frugiperda. Full crystallographic information is available from OCA.

Reference

A helical lid converts a sulfotransferase to a dehydratase., Pakhomova S, Kobayashi M, Buck J, Newcomer ME, Nat Struct Biol. 2001 May;8(5):447-51. PMID:11323722 Page seeded by OCA on Fri May 2 16:30:32 2008

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