1fng
From Proteopedia
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[[Image:1fng.gif|left|200px]] | [[Image:1fng.gif|left|200px]] | ||
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'''HISTOCOMPATIBILITY ANTIGEN''' | '''HISTOCOMPATIBILITY ANTIGEN''' | ||
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[[Category: Miley, M J.]] | [[Category: Miley, M J.]] | ||
[[Category: Nelson, C A.]] | [[Category: Nelson, C A.]] | ||
| - | [[Category: | + | [[Category: Histocompatibility antigen]] |
| - | [[Category: | + | [[Category: Immune system]] |
| - | [[Category: | + | [[Category: Mhc]] |
| - | [[Category: | + | [[Category: Peptide]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:32:12 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:32, 2 May 2008
HISTOCOMPATIBILITY ANTIGEN
Overview
To better understand TCR discrimination of multiple ligands, we have analyzed the crystal structures of two Hb peptide/I-E(k) complexes that differ by only a single amino acid substitution at the P6 anchor position within the peptide (E73D). Detailed comparison of multiple independently determined structures at 1.9 A resolution reveals that removal of a single buried methylene group can alter a critical portion of the TCR recognition surface. Significant variance was observed in the peptide P5-P8 main chain as well as a rotamer difference at LeuP8, approximately 10 A distal from the substitution. No significant variations were observed in the conformation of the two MHC class II molecules. The ligand alteration results in two peptide/MHC complexes that generate bulk T cell responses that are distinct and essentially nonoverlapping. For the Hb-specific T cell 3.L2, substitution reduces the potency of the ligand 1000-fold. Soluble 3.L2 TCR binds the two peptide/MHC complexes with similar affinity, although with faster kinetics. These results highlight the role of subtle variations in MHC Ag presentation on T cell activation and signaling.
About this Structure
1FNG is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural and functional consequences of altering a peptide MHC anchor residue., Kersh GJ, Miley MJ, Nelson CA, Grakoui A, Horvath S, Donermeyer DL, Kappler J, Allen PM, Fremont DH, J Immunol. 2001 Mar 1;166(5):3345-54. PMID:11207290 Page seeded by OCA on Fri May 2 16:32:12 2008
