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2b2d

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Revision as of 06:46, 10 November 2021

RNA stemloop operator from bacteriophage QBETA complexed with an N87S,E89K mutant MS2 capsid

Structural highlights

2b2d is a 5 chain structure with sequence from Bacteriophage ms2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2bny, 1aq3, 1aq4, 1bms, 1dzs, 1e6t, 2b2e, 2b2g
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[COAT_BPMS2] Forms the phage shell; binds to the phage RNA.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.

Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2.,Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG Structure. 2006 Mar;14(3):487-95. PMID:16531233^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG. Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2. Structure. 2006 Mar;14(3):487-95. PMID:16531233 doi:http://dx.doi.org/10.1016/j.str.2005.12.006

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2b2d"

@@ Line 3: / Line 3: @@
 <StructureSection load='2b2d' size='340' side='right'caption='[[2b2d]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2b2d]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2D FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2b2d]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B2D FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2bny|2bny]], [[1aq3|1aq3]], [[1aq4|1aq4]], [[1bms|1bms]], [[1dzs|1dzs]], [[1e6t|1e6t]], [[2b2e|2b2e]], [[2b2g|2b2g]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2bny|2bny]], [[1aq3|1aq3]], [[1aq4|1aq4]], [[1bms|1bms]], [[1dzs|1dzs]], [[1e6t|1e6t]], [[2b2e|2b2e]], [[2b2g|2b2g]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2d OCA], [http://pdbe.org/2b2d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b2d RCSB], [http://www.ebi.ac.uk/pdbsum/2b2d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2d ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2d OCA], [https://pdbe.org/2b2d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b2d RCSB], [https://www.ebi.ac.uk/pdbsum/2b2d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2d ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/COAT_BPMS2 COAT_BPMS2]] Forms the phage shell; binds to the phage RNA.
+[[https://www.uniprot.org/uniprot/COAT_BPMS2 COAT_BPMS2]] Forms the phage shell; binds to the phage RNA.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

2b2d

From Proteopedia

Revision as of 06:46, 10 November 2021

RNA stemloop operator from bacteriophage QBETA complexed with an N87S,E89K mutant MS2 capsid

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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