2eqd
From Proteopedia
(Difference between revisions)
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==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum== | ==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum== | ||
| - | <StructureSection load='2eqd' size='340' side='right'caption='[[2eqd]]' scene=''> | + | <StructureSection load='2eqd' size='340' side='right'caption='[[2eqd]], [[Resolution|resolution]] 2.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EQD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EQD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2eqd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"ruminiclostridium_thermocellum"_(viljoen_et_al._1926)_yutin_and_galperin_2013 "ruminiclostridium thermocellum" (viljoen et al. 1926) yutin and galperin 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EQD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EQD FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eqd OCA], [https://pdbe.org/2eqd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eqd RCSB], [https://www.ebi.ac.uk/pdbsum/2eqd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eqd ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2e4t|2e4t]], [[2e0p|2e0p]], [[2eex|2eex]], [[2ej1|2ej1]], [[2e07|2e07]]</div></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">celJ, celJ-cel44A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 "Ruminiclostridium thermocellum" (Viljoen et al. 1926) Yutin and Galperin 2013])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eqd OCA], [https://pdbe.org/2eqd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eqd RCSB], [https://www.ebi.ac.uk/pdbsum/2eqd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eqd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eqd ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eqd ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of Cel44A, which is one of the enzymatic components of the cellulosome of Clostridium thermocellum, was solved at a resolution of 0.96 A. This enzyme belongs to glycoside hydrolase family (GH family) 44. The structure reveals that Cel44A consists of a TIM-like barrel domain and a beta-sandwich domain. The wild-type and the E186Q mutant structures complexed with substrates suggest that two glutamic acid residues, Glu(186) and Glu(359), are the active residues of the enzyme. Biochemical experiments were performed to confirm this idea. The structural features indicate that GH family 44 belongs to clan GH-A and that the reaction catalyzed by Cel44A is retaining type hydrolysis. The stereochemical course of hydrolysis was confirmed by a (1)H NMR experiment using the reduced cellooligosaccharide as a substrate. | ||
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| + | Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum.,Kitago Y, Karita S, Watanabe N, Kamiya M, Aizawa T, Sakka K, Tanaka I J Biol Chem. 2007 Dec 7;282(49):35703-11. Epub 2007 Sep 28. PMID:17905739<ref>PMID:17905739</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2eqd" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Glucanase 3D structures|Glucanase 3D structures]] | *[[Glucanase 3D structures|Glucanase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Karita S]] | + | [[Category: Karita, S]] |
| - | [[Category: Kitago Y]] | + | [[Category: Kitago, Y]] |
| - | [[Category: Sakka K]] | + | [[Category: Sakka, K]] |
| - | [[Category: Tanaka I]] | + | [[Category: Tanaka, I]] |
| - | [[Category: Watanabe N]] | + | [[Category: Watanabe, N]] |
| + | [[Category: Beta-sandwich]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: The composite domain of gh family 5]] | ||
| + | [[Category: Tim-barrel]] | ||
| + | [[Category: Tim-like barrel]] | ||
Revision as of 06:57, 10 November 2021
Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum
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