1cll

From Proteopedia

---

Revision as of 14:16, 12 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1cll, resolution 1.7Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CALMODULIN STRUCTURE REFINED AT 1.7 ANGSTROMS RESOLUTION

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

We have determined and refined the crystal structure of a recombinant, calmodulin at 1.7 A resolution. The structure was determined by molecular, replacement, using the 2.2 A published native bovine brain structure as, the starting model. The final crystallographic R-factor, using 14,469, reflections in the 10.0 to 1.7 A range with structure factors exceeding, 0.5 sigma, is 0.216. Bond lengths and bond angle distances have, root-mean-square deviations from ideal values of 0.009 A and 0.032 A, respectively. The final model consists of 1279 non-hydrogen atoms, including four calcium ions, 1130 protein atoms, including three Asp118, side-chain atoms in double conformation, 139 water molecules and one, ethanol molecule. The electron densities for residues 1 to 4 and 148 of, calmodulin are poorly defined, and not included in our model, except for, main-chain atoms of residue 4. The calmodulin structure from our crystals, is very similar to the earlier 2.2 A structure described by Babu and, coworkers with a root-mean-square deviation of 0.36 A. Calmodulin remains, a dumb-bell-shaped molecule, with similar lobes and connected by a central, alpha-helix. Each lobe contains three alpha-helices and two Ca2+ binding, EF hand loops, with a short antiparallel beta-sheet between adjacent EF, hand loops and one non-EF hand loop. There are some differences in the, structure of the central helix. The crystal packing is extensively, studied, and facile crystal growth along the z-axis of the triclinic, crystals is explained. Herein, we describe hydrogen bonding in the various, secondary structure elements and hydration of calmodulin.

Disease

Known diseases associated with this structure: Cavernous malformations of CNS and retina OMIM:[604214], Cerebral cavernous malformations-1 OMIM:[604214], Hyperkeratotic cutaneous capillary-venous malformations associated with cerebral capillary malformations OMIM:[604214], Leukemia, acute T-cell lymphoblastic OMIM:[603025], Leukemia, acute myeloid OMIM:[603025]

About this Structure

1CLL is a Single protein structure of sequence from Homo sapiens with CA and EOH as ligands. The following page contains interesting information on the relation of 1CLL with [Calmodulin]. Full crystallographic information is available from OCA.

Reference

Calmodulin structure refined at 1.7 A resolution., Chattopadhyaya R, Meador WE, Means AR, Quiocho FA, J Mol Biol. 1992 Dec 20;228(4):1177-92. PMID:1474585

Page seeded by OCA on Mon Nov 12 16:23:02 2007