3die

From Proteopedia

(Difference between revisions)

Revision as of 07:32, 10 November 2021

Domain swapping of Staphylococcus Aureus thioredoxin W28A mutant

Structural highlights

3die is a 2 chain structure with sequence from "micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	2o7k, 2o89, 2o87, 2o85
Gene:	trxA ("Micrococcus aureus" (Rosenbach 1884) Zopf 1885)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The thioredoxin (Trx) fold is a small monomeric domain that is ubiquitous in redox-active enzymes. Trxs are characterized by a typical WCGPC active-site sequence motif. A single active-site mutation of the tryptophan to an alanine in Staphylococcus aureus Trx converts the oxidized protein into a biologically inactive domain-swapped dimer. While the monomeric protein unfolds reversibly in a two-state manner, the oxidized dimeric form is kinetically stable and converts to the monomeric form upon refolding. After reduction, the half-life of the dimer decreases many orders of magnitude to approximately 4.3 h, indicating that the active-site disulfide between Cys29 and Cys32 is an important determinant for the kinetics of unfolding. We propose kinetic stability as a possible evolutionary strategy in the evolution of multimeric proteins from their monomeric ancestors by domain swapping, which, for this biologically inactive Trx mutant, turned out to be an evolutionary dead end.

Coupling of domain swapping to kinetic stability in a thioredoxin mutant.,Garcia-Pino A, Martinez-Rodriguez S, Wahni K, Wyns L, Loris R, Messens J J Mol Biol. 2009 Feb 6;385(5):1590-9. Epub 2008 Dec 3. PMID:19071139^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Garcia-Pino A, Martinez-Rodriguez S, Wahni K, Wyns L, Loris R, Messens J. Coupling of domain swapping to kinetic stability in a thioredoxin mutant. J Mol Biol. 2009 Feb 6;385(5):1590-9. Epub 2008 Dec 3. PMID:19071139 doi:10.1016/j.jmb.2008.11.040

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3die"

@@ Line 1: / Line 1: @@
 ==Domain swapping of Staphylococcus Aureus thioredoxin W28A mutant==
-<StructureSection load='3die' size='340' side='right' caption='[[3die]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+<StructureSection load='3die' size='340' side='right'caption='[[3die]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3die]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DIE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DIE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3die]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DIE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DIE FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2o7k|2o7k]], [[2o89|2o89]], [[2o87|2o87]], [[2o85|2o85]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2o7k|2o7k]], [[2o89|2o89]], [[2o87|2o87]], [[2o85|2o85]]</div></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">trxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">trxA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3die FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3die OCA], [http://pdbe.org/3die PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3die RCSB], [http://www.ebi.ac.uk/pdbsum/3die PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3die ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3die FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3die OCA], [https://pdbe.org/3die PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3die RCSB], [https://www.ebi.ac.uk/pdbsum/3die PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3die ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 30: / Line 30: @@
 ==See Also==
-*[[Thioredoxin|Thioredoxin]]
+*[[Thioredoxin 3D structures|Thioredoxin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Loris, R]]
 [[Category: Martinez-Rodriguez, S]]

3die

From Proteopedia

Revision as of 07:32, 10 November 2021

Domain swapping of Staphylococcus Aureus thioredoxin W28A mutant

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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