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3fk7
From Proteopedia
(Difference between revisions)
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==Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc== | ==Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc== | ||
| - | <StructureSection load='3fk7' size='340' side='right' caption='[[3fk7]], [[Resolution|resolution]] 2.06Å' scene=''> | + | <StructureSection load='3fk7' size='340' side='right'caption='[[3fk7]], [[Resolution|resolution]] 2.06Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3fk7]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3fk7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FK7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4DM:(4AS,12AS)-3,10,11,12A-TETRAHYDROXY-6-METHYL-1,12-DIOXO-1,4,4A,5,12,12A-HEXAHYDROTETRACENE-2-CARBOXAMIDE'>4DM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4DM:(4AS,12AS)-3,10,11,12A-TETRAHYDROXY-6-METHYL-1,12-DIOXO-1,4,4A,5,12,12A-HEXAHYDROTETRACENE-2-CARBOXAMIDE'>4DM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vke|2vke]], [[2o7o|2o7o]], [[2vkv|2vkv]], [[2tct|2tct]], [[3fk6|3fk6]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vke|2vke]], [[2o7o|2o7o]], [[2vkv|2vkv]], [[2tct|2tct]], [[3fk6|3fk6]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tetR ([ | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tetR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk7 OCA], [https://pdbe.org/3fk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fk7 RCSB], [https://www.ebi.ac.uk/pdbsum/3fk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fk7 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[ | + | [[https://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites. |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/3fk7_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/3fk7_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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</div> | </div> | ||
<div class="pdbe-citations 3fk7" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 3fk7" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Bacillus coli migula 1895]] | [[Category: Bacillus coli migula 1895]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Gmeiner, P]] | [[Category: Gmeiner, P]] | ||
[[Category: Hillen, W]] | [[Category: Hillen, W]] | ||
Revision as of 07:39, 10 November 2021
Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc
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Categories: Bacillus coli migula 1895 | Large Structures | Gmeiner, P | Hillen, W | Klieber, M A | Lochner, S | Muller, Y A | Scholz, O | 4-de-dimethylamino-anhydrotetracycline | Altered inducer specificity | Antibiotic resistance | Bacterial transcription regulation | Dna-binding | Magnesium | Metal-binding | Repressor | Tetracycline repressor | Transcription | Transcription regulation | Transposable element
