|
|
| Line 1: |
Line 1: |
| | | | |
| | ==crystal structure of effect protein AvrptoB complexed with kinase Pto== | | ==crystal structure of effect protein AvrptoB complexed with kinase Pto== |
| - | <StructureSection load='3hgk' size='340' side='right' caption='[[3hgk]], [[Resolution|resolution]] 3.30Å' scene=''> | + | <StructureSection load='3hgk' size='340' side='right'caption='[[3hgk]], [[Resolution|resolution]] 3.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3hgk]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"pseudomonas_tomato"_(okabe_1933)_alstatt_1944 "pseudomonas tomato" (okabe 1933) alstatt 1944] and [http://en.wikipedia.org/wiki/Currant_tomato Currant tomato]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HGK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3hgk]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/"pseudomonas_tomato"_(okabe_1933)_alstatt_1944 "pseudomonas tomato" (okabe 1933) alstatt 1944] and [https://en.wikipedia.org/wiki/Currant_tomato Currant tomato]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HGK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HGK FirstGlance]. <br> |
| | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr> | | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hgl|3hgl]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3hgl|3hgl]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Pto ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4084 Currant tomato]), hopAB2, avrPtoB, PSPTO_3087 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=323 "Pseudomonas tomato" (Okabe 1933) Alstatt 1944])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Pto ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4084 Currant tomato]), hopAB2, avrPtoB, PSPTO_3087 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=323 "Pseudomonas tomato" (Okabe 1933) Alstatt 1944])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hgk OCA], [http://pdbe.org/3hgk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3hgk RCSB], [http://www.ebi.ac.uk/pdbsum/3hgk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3hgk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hgk OCA], [https://pdbe.org/3hgk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hgk RCSB], [https://www.ebi.ac.uk/pdbsum/3hgk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hgk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HPAB2_PSESM HPAB2_PSESM]] Effector protein involved in gene-for-gene resistance in tomato plants. It is recognized by the host Pto resistance protein and elicits Pto and Prf-dependent hypersensitive response (HR) and programmed cell death (PCD), resulting in host immunity. In susceptible plants, acts as a virulence factor by suppressing PCD and HR-based plant immunity. This function requires its E3 ubiquitin ligase activity probably by recruiting E2 enzymes and transferring ubiquitin molecules to cellular proteins involved in regulation of PCD and targeting them for degradation. Also, induces expression of host genes involved in ethylene biosynthesis and signaling, in particular ACO1 and ACO2, encoding the ethylene-forming enzyme ACC oxidase.<ref>PMID:12062102</ref> <ref>PMID:12505984</ref> <ref>PMID:16167902</ref> <ref>PMID:16477026</ref> <ref>PMID:16373536</ref> | + | [[https://www.uniprot.org/uniprot/HPAB2_PSESM HPAB2_PSESM]] Effector protein involved in gene-for-gene resistance in tomato plants. It is recognized by the host Pto resistance protein and elicits Pto and Prf-dependent hypersensitive response (HR) and programmed cell death (PCD), resulting in host immunity. In susceptible plants, acts as a virulence factor by suppressing PCD and HR-based plant immunity. This function requires its E3 ubiquitin ligase activity probably by recruiting E2 enzymes and transferring ubiquitin molecules to cellular proteins involved in regulation of PCD and targeting them for degradation. Also, induces expression of host genes involved in ethylene biosynthesis and signaling, in particular ACO1 and ACO2, encoding the ethylene-forming enzyme ACC oxidase.<ref>PMID:12062102</ref> <ref>PMID:12505984</ref> <ref>PMID:16167902</ref> <ref>PMID:16477026</ref> <ref>PMID:16373536</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 32: |
Line 32: |
| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Avirulence protein|Avirulence protein]] | + | *[[Avirulence protein 3D structures|Avirulence protein 3D structures]] |
| - | *[[Serine/threonine protein kinase|Serine/threonine protein kinase]] | + | *[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| Line 39: |
Line 39: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Currant tomato]] | | [[Category: Currant tomato]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Chai, J]] | | [[Category: Chai, J]] |
| | [[Category: Dong, J]] | | [[Category: Dong, J]] |
| Structural highlights
Function
[HPAB2_PSESM] Effector protein involved in gene-for-gene resistance in tomato plants. It is recognized by the host Pto resistance protein and elicits Pto and Prf-dependent hypersensitive response (HR) and programmed cell death (PCD), resulting in host immunity. In susceptible plants, acts as a virulence factor by suppressing PCD and HR-based plant immunity. This function requires its E3 ubiquitin ligase activity probably by recruiting E2 enzymes and transferring ubiquitin molecules to cellular proteins involved in regulation of PCD and targeting them for degradation. Also, induces expression of host genes involved in ethylene biosynthesis and signaling, in particular ACO1 and ACO2, encoding the ethylene-forming enzyme ACC oxidase.[1] [2] [3] [4] [5]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Resistance to bacterial speck disease in tomato (Solanum lycopersicum) is activated upon recognition by the host Pto kinase of either one of two sequence-unrelated effector proteins, AvrPto or AvrPtoB, from Pseudomonas syringae pv tomato (Pst). Pto induces Pst immunity by acting in concert with the Prf protein. The recently reported structure of the AvrPto-Pto complex revealed that interaction of AvrPto with Pto appears to relieve an inhibitory effect of Pto, allowing Pto to activate Prf. Here, we present the crystal structure of the Pto binding domain of AvrPtoB (residues 121 to 205) at a resolution of 1.9A and of the AvrPtoB(121-205)-Pto complex at a resolution of 3.3 A. AvrPtoB(121-205) exhibits a tertiary fold that is completely different from that of AvrPto, and its conformation remains largely unchanged upon binding to Pto. In common with AvrPto-Pto, the AvrPtoB-Pto complex relies on two interfaces. One of these interfaces is similar in both complexes, although the primary amino acid sequences from the two effector proteins are very different. Amino acid substitutions in Pto at the other interface disrupt the interaction of AvrPtoB-Pto but not that of AvrPto-Pto. Interestingly, substitutions in Pto affecting this unique interface also cause Pto to induce Prf-dependent host cell death independently of either effector protein.
Crystal structure of the complex between Pseudomonas effector AvrPtoB and the tomato Pto kinase reveals both a shared and a unique interface compared with AvrPto-Pto.,Dong J, Xiao F, Fan F, Gu L, Cang H, Martin GB, Chai J Plant Cell. 2009 Jun;21(6):1846-59. Epub 2009 Jun 9. PMID:19509331[6]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kim YJ, Lin NC, Martin GB. Two distinct Pseudomonas effector proteins interact with the Pto kinase and activate plant immunity. Cell. 2002 May 31;109(5):589-98. PMID:12062102
- ↑ Abramovitch RB, Kim YJ, Chen S, Dickman MB, Martin GB. Pseudomonas type III effector AvrPtoB induces plant disease susceptibility by inhibition of host programmed cell death. EMBO J. 2003 Jan 2;22(1):60-9. PMID:12505984 doi:http://dx.doi.org/10.1093/emboj/cdg006
- ↑ Cohn JR, Martin GB. Pseudomonas syringae pv. tomato type III effectors AvrPto and AvrPtoB promote ethylene-dependent cell death in tomato. Plant J. 2005 Oct;44(1):139-54. PMID:16167902 doi:http://dx.doi.org/TPJ2516
- ↑ Abramovitch RB, Janjusevic R, Stebbins CE, Martin GB. Type III effector AvrPtoB requires intrinsic E3 ubiquitin ligase activity to suppress plant cell death and immunity. Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2851-6. Epub 2006 Feb 13. PMID:16477026 doi:http://dx.doi.org/0507892103
- ↑ Janjusevic R, Abramovitch RB, Martin GB, Stebbins CE. A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase. Science. 2006 Jan 13;311(5758):222-6. Epub 2005 Dec 22. PMID:16373536 doi:http://dx.doi.org/1120131
- ↑ Dong J, Xiao F, Fan F, Gu L, Cang H, Martin GB, Chai J. Crystal structure of the complex between Pseudomonas effector AvrPtoB and the tomato Pto kinase reveals both a shared and a unique interface compared with AvrPto-Pto. Plant Cell. 2009 Jun;21(6):1846-59. Epub 2009 Jun 9. PMID:19509331 doi:10.1105/tpc.109.066878
|
