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| | <StructureSection load='3ity' size='340' side='right'caption='[[3ity]], [[Resolution|resolution]] 1.84Å' scene=''> | | <StructureSection load='3ity' size='340' side='right'caption='[[3ity]], [[Resolution|resolution]] 1.84Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ity]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_sewerinii"_bergey_et_al._1923 "achromobacter sewerinii" bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ITY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3ITY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ity]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"achromobacter_sewerinii"_bergey_et_al._1923 "achromobacter sewerinii" bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ITY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ITY FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2hcv|2hcv]], [[2i56|2i56]], [[2i57|2i57]], [[3itl|3itl]], [[3ito|3ito]], [[3itt|3itt]], [[3itv|3itv]], [[3itx|3itx]], [[3iud|3iud]], [[3iuh|3iuh]], [[3iui|3iui]]</td></tr> | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2hcv|2hcv]], [[2i56|2i56]], [[2i57|2i57]], [[3itl|3itl]], [[3ito|3ito]], [[3itt|3itt]], [[3itv|3itv]], [[3itx|3itx]], [[3iud|3iud]], [[3iuh|3iuh]], [[3iui|3iui]]</div></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-rhamnose_isomerase L-rhamnose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.14 5.3.1.14] </span></td></tr> | + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/L-rhamnose_isomerase L-rhamnose isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.14 5.3.1.14] </span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3ity FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ity OCA], [http://pdbe.org/3ity PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ity RCSB], [http://www.ebi.ac.uk/pdbsum/3ity PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ity ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ity FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ity OCA], [https://pdbe.org/3ity PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ity RCSB], [https://www.ebi.ac.uk/pdbsum/3ity PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ity ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| Structural highlights
3ity is a 4 chain structure with sequence from "achromobacter_sewerinii"_bergey_et_al._1923 "achromobacter sewerinii" bergey et al. 1923. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Related: | 2hcv, 2i56, 2i57, 3itl, 3ito, 3itt, 3itv, 3itx, 3iud, 3iuh, 3iui |
| Activity: | L-rhamnose isomerase, with EC number 5.3.1.14 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
L-Rhamnose isomerase (L-RhI) catalyzes the reversible isomerization of L-rhamnose to L-rhamnulose. Pseudomonas stutzeril-RhI, with a broad substrate specificity, can catalyze not only the isomerization of L-rhamnose, but also that between D-allose and D-psicose. For the aldose-ketose isomerization by L-RhI, a metal-mediated hydride-shift mechanism has been proposed, but the catalytic mechanism is still not entirely understood. To elucidate the entire reaction mechanism, the X-ray structures of P. stutzeril-RhI in an Mn(2+)-bound form, and of two inactive mutant forms of P. stutzeril-RhI (S329K and D327N) in a complex with substrate/product, were determined. The structure of the Mn(2+)-bound enzyme indicated that the catalytic site interconverts between two forms with the displacement of the metal ion to recognize both pyranose and furanose ring substrates. Solving the structures of S329K-substrates allowed us to examine the metal-mediated hydride-shift mechanism of L-RhI in detail. The structural analysis of D327N-substrates and additional modeling revealed Asp327 to be responsible for the ring opening of furanose, and a water molecule coordinating with the metal ion to be involved in the ring opening of pyranose.
Catalytic reaction mechanism of Pseudomonas stutzeri L-rhamnose isomerase deduced from X-ray structures.,Yoshida H, Yamaji M, Ishii T, Izumori K, Kamitori S FEBS J. 2010 Feb;277(4):1045-57. Epub 2010 Jan 20. PMID:20088877[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Yoshida H, Yamaji M, Ishii T, Izumori K, Kamitori S. Catalytic reaction mechanism of Pseudomonas stutzeri L-rhamnose isomerase deduced from X-ray structures. FEBS J. 2010 Feb;277(4):1045-57. Epub 2010 Jan 20. PMID:20088877 doi:10.1111/j.1742-4658.2009.07548.x
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