6ypi
From Proteopedia
(Difference between revisions)
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==Structure of the engineered metallo-Diels-Alderase DA7 W16G,K58Q,L77R,T78R== | ==Structure of the engineered metallo-Diels-Alderase DA7 W16G,K58Q,L77R,T78R== | ||
| - | <StructureSection load='6ypi' size='340' side='right'caption='[[6ypi]]' scene=''> | + | <StructureSection load='6ypi' size='340' side='right'caption='[[6ypi]], [[Resolution|resolution]] 1.48Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YPI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ypi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YPI FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ypi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ypi OCA], [https://pdbe.org/6ypi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ypi RCSB], [https://www.ebi.ac.uk/pdbsum/6ypi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ypi ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=DMX:3-[BENZYL(DIMETHYL)AMMONIO]PROPANE-1-SULFONATE'>DMX</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ypi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ypi OCA], [https://pdbe.org/6ypi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ypi RCSB], [https://www.ebi.ac.uk/pdbsum/6ypi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ypi ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | New enzyme catalysts are usually engineered by repurposing the active sites of natural proteins. Here we show that design and directed evolution can be used to transform a non-natural, functionally naive zinc-binding protein into a highly active catalyst for an abiological hetero-Diels-Alder reaction. The artificial metalloenzyme achieves >10(4) turnovers per active site, exerts absolute control over reaction pathway and product stereochemistry, and displays a catalytic proficiency (1/KTS = 2.9 x 10(10) M(-1)) that exceeds all previously characterized Diels-Alderases. These properties capitalize on effective Lewis acid catalysis, a chemical strategy for accelerating Diels-Alder reactions common in the laboratory but so far unknown in nature. Extension of this approach to other metal ions and other de novo scaffolds may propel the design field in exciting new directions. | ||
| + | |||
| + | Efficient Lewis acid catalysis of an abiological reaction in a de novo protein scaffold.,Basler S, Studer S, Zou Y, Mori T, Ota Y, Camus A, Bunzel HA, Helgeson RC, Houk KN, Jimenez-Oses G, Hilvert D Nat Chem. 2021 Mar;13(3):231-235. doi: 10.1038/s41557-020-00628-4. Epub 2021 Feb , 1. PMID:33526894<ref>PMID:33526894</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6ypi" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Basler S]] | + | [[Category: Synthetic construct sequences]] |
| - | [[Category: Hilvert D]] | + | [[Category: Basler, S]] |
| - | [[Category: Mori T]] | + | [[Category: Hilvert, D]] |
| + | [[Category: Mori, T]] | ||
| + | [[Category: De novo diels-alderase]] | ||
| + | [[Category: De novo protein]] | ||
Revision as of 07:56, 10 November 2021
Structure of the engineered metallo-Diels-Alderase DA7 W16G,K58Q,L77R,T78R
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