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1fof
From Proteopedia
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[[Image:1fof.gif|left|200px]] | [[Image:1fof.gif|left|200px]] | ||
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'''CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-10''' | '''CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-10''' | ||
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[[Category: Page, M G.P.]] | [[Category: Page, M G.P.]] | ||
[[Category: Strynadka, N C.J.]] | [[Category: Strynadka, N C.J.]] | ||
| - | [[Category: | + | [[Category: Beta-lactamase]] |
| - | [[Category: | + | [[Category: Class-d]] |
| - | [[Category: | + | [[Category: Cobalt]] |
| - | [[Category: | + | [[Category: Oxa-10]] |
| - | [[Category: | + | [[Category: Oxacillinase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:34:26 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:34, 2 May 2008
CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-10
Overview
We report the crystal structure of a class D beta-lactamase, the broad spectrum enzyme OXA-10 from Pseudomonas aeruginosa at 2.0 A resolution. There are significant differences between the overall fold observed in this structure and those of the evolutionarily related class A and class C beta-lactamases. Furthermore, the structure suggests the unique, cation mediated formation of a homodimer. Kinetic and hydrodynamic data shows that the dimer is a relevant species in solution and is the more active form of the enzyme. Comparison of the molecular details of the active sites of the class A and class C enzymes with the OXA-10 structure reveals that there is no counterpart in OXA-10 to the residues proposed to act as general bases in either of these enzymes (Glu 166 and Tyr 150, respectively). Our structures of the native and chloride inhibited forms of OXA-10 suggest that the class D enzymes have evolved a distinct catalytic mechanism for beta-lactam hydrolysis. Clinical variants of OXA-10 are also discussed in light of the structure.
About this Structure
1FOF is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
Crystal structure of the class D beta-lactamase OXA-10., Paetzel M, Danel F, de Castro L, Mosimann SC, Page MG, Strynadka NC, Nat Struct Biol. 2000 Oct;7(10):918-25. PMID:11017203 Page seeded by OCA on Fri May 2 16:34:26 2008
