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2xgf

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Revision as of 08:40, 10 November 2021

Structure of the bacteriophage T4 long tail fibre needle-shaped receptor-binding tip

Structural highlights

2xgf is a 3 chain structure with sequence from Bpt4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FIB37_BPT4] Structural component of the distal-half of the long-tail fiber. It constitutes the part of the long-tail fibers that recognizes the bacterial receptor. The long-tail fiber of T4 is about 1600 Angstroms long with a kink in the middle that divides the fiber into proximal and distal halves. The thin tip of the distal half-fiber interacts with the bacterial lipopolysaccharide receptor and specifies the host range of the phage.^[1]

Publication Abstract from PubMed

Bacteriophages are the most numerous organisms in the biosphere. In spite of their biological significance and the spectrum of potential applications, little high-resolution structural detail is available on their receptor-binding fibers. Here we present the crystal structure of the receptor-binding tip of the bacteriophage T4 long tail fiber, which is highly homologous to the tip of the bacteriophage lambda side tail fibers. This structure reveals an unusual elongated six-stranded antiparallel beta-strand needle domain containing seven iron ions coordinated by histidine residues arranged colinearly along the core of the biological unit. At the end of the tip, the three chains intertwine forming a broader head domain, which contains the putative receptor interaction site. The structure reveals a previously unknown beta-structured fibrous fold, provides insights into the remarkable stability of the fiber, and suggests a framework for mutations to expand or modulate receptor-binding specificity.

Structure of the bacteriophage T4 long tail fiber receptor-binding tip.,Bartual SG, Otero JM, Garcia-Doval C, Llamas-Saiz AL, Kahn R, Fox GC, van Raaij MJ Proc Natl Acad Sci U S A. 2010 Nov 1. PMID:21041684^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cerritelli ME, Wall JS, Simon MN, Conway JF, Steven AC. Stoichiometry and domainal organization of the long tail-fiber of bacteriophage T4: a hinged viral adhesin. J Mol Biol. 1996 Aug 2;260(5):767-80. PMID:8709154 doi:http://dx.doi.org/10.1006/jmbi.1996.0436
↑ Bartual SG, Otero JM, Garcia-Doval C, Llamas-Saiz AL, Kahn R, Fox GC, van Raaij MJ. Structure of the bacteriophage T4 long tail fiber receptor-binding tip. Proc Natl Acad Sci U S A. 2010 Nov 1. PMID:21041684 doi:10.1073/pnas.1011218107

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xgf"

@@ Line 3: / Line 3: @@
 <StructureSection load='2xgf' size='340' side='right'caption='[[2xgf]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xgf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XGF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xgf]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XGF FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xgf OCA], [http://pdbe.org/2xgf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xgf RCSB], [http://www.ebi.ac.uk/pdbsum/2xgf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xgf ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xgf OCA], [https://pdbe.org/2xgf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xgf RCSB], [https://www.ebi.ac.uk/pdbsum/2xgf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xgf ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/FIB37_BPT4 FIB37_BPT4]] Structural component of the distal-half of the long-tail fiber. It constitutes the part of the long-tail fibers that recognizes the bacterial receptor. The long-tail fiber of T4 is about 1600 Angstroms long with a kink in the middle that divides the fiber into proximal and distal halves. The thin tip of the distal half-fiber interacts with the bacterial lipopolysaccharide receptor and specifies the host range of the phage.<ref>PMID:8709154</ref>
+[[https://www.uniprot.org/uniprot/FIB37_BPT4 FIB37_BPT4]] Structural component of the distal-half of the long-tail fiber. It constitutes the part of the long-tail fibers that recognizes the bacterial receptor. The long-tail fiber of T4 is about 1600 Angstroms long with a kink in the middle that divides the fiber into proximal and distal halves. The thin tip of the distal half-fiber interacts with the bacterial lipopolysaccharide receptor and specifies the host range of the phage.<ref>PMID:8709154</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

2xgf

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Revision as of 08:40, 10 November 2021

Structure of the bacteriophage T4 long tail fibre needle-shaped receptor-binding tip

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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