7ayx
From Proteopedia
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==Structure of the cytochrome P450 AryC from Streptomyces roseosporus NRRL 15998== | ==Structure of the cytochrome P450 AryC from Streptomyces roseosporus NRRL 15998== | ||
| - | <StructureSection load='7ayx' size='340' side='right'caption='[[7ayx]]' scene=''> | + | <StructureSection load='7ayx' size='340' side='right'caption='[[7ayx]], [[Resolution|resolution]] 2.53Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AYX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ayx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AYX FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ayx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ayx OCA], [https://pdbe.org/7ayx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ayx RCSB], [https://www.ebi.ac.uk/pdbsum/7ayx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ayx ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ayx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ayx OCA], [https://pdbe.org/7ayx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ayx RCSB], [https://www.ebi.ac.uk/pdbsum/7ayx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ayx ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The arylomycin antibiotics are potent inhibitors of bacterial type I signal peptidase. These lipohexapeptides contain a biaryl structural motif reminiscent of glycopeptide antibiotics. We herein describe the functional and structural evaluation of AryC, the cytochrome P450 performing biaryl coupling in biosynthetic arylomycin assembly. Unlike its enzymatic counterparts in glycopeptide biosynthesis, AryC converts free substrates without the requirement of any protein interaction partner, likely enabled by a strongly hydrophobic cavity at the surface of AryC pointing to the substrate tunnel. This activity enables chemo-enzymatic assembly of arylomycin A2 that combines the advantages of liquid- and solid-phase peptide synthesis with late-stage enzymatic cross coupling. The reactivity of AryC is unprecedented in cytochrome P450-mediated biaryl construction in non-ribosomal peptides, in which peptidyl carrier protein (PCP)-tethering so far was shown crucial both in vivo and in vitro. | ||
| + | |||
| + | Carrier Protein-Free Enzymatic Biaryl Coupling in Arylomycin A2 Assembly and Structure of the Cytochrome P450 AryC.,Aldemir H, Shu S, Schaefers F, Hong H, Richarz R, Harteis S, Einsiedler M, Milzarek TM, Schneider S, Gulder TAM Chemistry. 2021 Nov 2. doi: 10.1002/chem.202103389. PMID:34725865<ref>PMID:34725865</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ayx" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Gulder | + | [[Category: Gulder, T A.M]] |
| - | [[Category: Schaefers F]] | + | [[Category: Schaefers, F]] |
| - | [[Category: Schneider S]] | + | [[Category: Schneider, S]] |
| + | [[Category: Arylomycin biosynthesis]] | ||
| + | [[Category: Cytc p450]] | ||
| + | [[Category: Cytosolic protein]] | ||
| + | [[Category: Oxidase]] | ||
Revision as of 14:09, 17 November 2021
Structure of the cytochrome P450 AryC from Streptomyces roseosporus NRRL 15998
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