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2a65

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Revision as of 14:36, 17 November 2021

Crystal structure of LEUTAA, a bacterial homolog of Na+/Cl--dependent neurotransmitter transporters

Structural highlights

2a65 is a 1 chain structure with sequence from Aquae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Na+/Cl--dependent transporters terminate synaptic transmission by using electrochemical gradients to drive the uptake of neurotransmitters, including the biogenic amines, from the synapse to the cytoplasm of neurons and glia. These transporters are the targets of therapeutic and illicit compounds, and their dysfunction has been implicated in multiple diseases of the nervous system. Here we present the crystal structure of a bacterial homologue of these transporters from Aquifex aeolicus, in complex with its substrate, leucine, and two sodium ions. The protein core consists of the first ten of twelve transmembrane segments, with segments 1-5 related to 6-10 by a pseudo-two-fold axis in the membrane plane. Leucine and the sodium ions are bound within the protein core, halfway across the membrane bilayer, in an occluded site devoid of water. The leucine and ion binding sites are defined by partially unwound transmembrane helices, with main-chain atoms and helix dipoles having key roles in substrate and ion binding. The structure reveals the architecture of this important class of transporter, illuminates the determinants of substrate binding and ion selectivity, and defines the external and internal gates.

Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters.,Yamashita A, Singh SK, Kawate T, Jin Y, Gouaux E Nature. 2005 Sep 8;437(7056):215-23. Epub 2005 Jul 24. PMID:16041361^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yamashita A, Singh SK, Kawate T, Jin Y, Gouaux E. Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters. Nature. 2005 Sep 8;437(7056):215-23. Epub 2005 Jul 24. PMID:16041361 doi:10.1038/nature03978

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2a65"

@@ Line 3: / Line 3: @@
 <StructureSection load='2a65' size='340' side='right'caption='[[2a65]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2a65]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquae Aquae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A65 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A65 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2a65]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquae Aquae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A65 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a65 OCA], [http://pdbe.org/2a65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a65 RCSB], [http://www.ebi.ac.uk/pdbsum/2a65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2a65 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a65 OCA], [https://pdbe.org/2a65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a65 RCSB], [https://www.ebi.ac.uk/pdbsum/2a65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a65 ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 28: / Line 28: @@
 ==See Also==
-*[[Suggestions for new articles|Suggestions for new articles]]
 *[[Symporter 3D structures|Symporter 3D structures]]
 == References ==

2a65

From Proteopedia

Revision as of 14:36, 17 November 2021

Crystal structure of LEUTAA, a bacterial homolog of Na+/Cl--dependent neurotransmitter transporters

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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