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2azo

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Revision as of 14:46, 17 November 2021

DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI

Structural highlights

2azo is a 2 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MUTH_ECOLI] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair.[HAMAP-Rule:MF_00759]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

MutS, MutL and MutH are the three essential proteins for initiation of methyl-directed DNA mismatch repair to correct mistakes made during DNA replication in Escherichia coli. MutH cleaves a newly synthesized and unmethylated daughter strand 5' to the sequence d(GATC) in a hemi-methylated duplex. Activation of MutH requires the recognition of a DNA mismatch by MutS and MutL. We have crystallized MutH in two space groups and solved the structures at 1.7 and 2.3 A resolution, respectively. The active site of MutH is located at an interface between two subdomains that pivot relative to one another, as revealed by comparison of the crystal structures, and this presumably regulates the nuclease activity. The relative motion of the two subdomains in MutH correlates with the position of a protruding C-terminal helix. This helix appears to act as a molecular lever through which MutS and MutL may communicate the detection of a DNA mismatch and activate MutH. With sequence homology to Sau3AI and structural similarity to PvuII endonuclease, MutH is clearly related to these enzymes by divergent evolution, and this suggests that type II restriction endonucleases evolved from a common ancestor.

Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases.,Ban C, Yang W EMBO J. 1998 Mar 2;17(5):1526-34. PMID:9482749^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ban C, Yang W. Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases. EMBO J. 1998 Mar 2;17(5):1526-34. PMID:9482749 doi:10.1093/emboj/17.5.1526

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2azo"

@@ Line 3: / Line 3: @@
 <StructureSection load='2azo' size='340' side='right'caption='[[2azo]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2azo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AZO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2azo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AZO FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2azo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2azo OCA], [http://pdbe.org/2azo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2azo RCSB], [http://www.ebi.ac.uk/pdbsum/2azo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2azo ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2azo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2azo OCA], [https://pdbe.org/2azo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2azo RCSB], [https://www.ebi.ac.uk/pdbsum/2azo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2azo ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MUTH_ECOLI MUTH_ECOLI]] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair.[HAMAP-Rule:MF_00759]
+[[https://www.uniprot.org/uniprot/MUTH_ECOLI MUTH_ECOLI]] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair.[HAMAP-Rule:MF_00759]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

2azo

From Proteopedia

Revision as of 14:46, 17 November 2021

DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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