Nitroreductase

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== Function ==
== Function ==
-
'''Nitroreductase''' (NR) is involved in the reduction of nitrogen-containing compounds including those containing NO<sub>2</sub> group. The NO<sub>2</sub> group is reduced to hydroxylamine<ref>PMID:11491290</ref>. NR uses FMN as cofactor. Among NRs are the oxygen-insensitive NAD(P)H NR and NADH dehydrogenase.
+
'''Nitroreductase''' or '''oxygen-insensitive NAD(P)H nitroreductase''' (NR) is involved in the reduction of nitrogen-containing compounds including those containing NO<sub>2</sub> group. The NO<sub>2</sub> group is reduced to hydroxylamine<ref>PMID:11491290</ref>. NR uses FMN as cofactor. Among NRs are the oxygen-insensitive NAD(P)H NR and NADH dehydrogenase.
== Relevance ==
== Relevance ==
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*Nitroreductase
*Nitroreductase
-
**[[1f5v ]], [[1oo5]] – EcNR – ''Escherichia coli''<br />
+
**[[1f5v ]], [[1oo5]] – EcNR + FMN – ''Escherichia coli''<br />
-
**[[3x21]], [[3x22]] – EcNR (mutant)<br />
+
**[[3x21]], [[3x22]] – EcNR (mutant) + FMN<br />
-
**[[3bm1]], [[1ds7]] – EcNR minimal<br />
+
**[[3bm1]], [[1ds7]] – EcNR minimal + FMN<br />
**[[3bm2]] – EcNR minimal -FMN<br />
**[[3bm2]] – EcNR minimal -FMN<br />
-
**[[1nec]], [[1kqd]] – EclNR – ''Enterobacter cloacae''<br />
+
**[[1nec]], [[1kqd]] – EclNR + FMN – ''Enterobacter cloacae''<br />
-
**[[1ywq]], [[2i7h]] – NR – ''Bacillus cereus''<br />
+
**[[1ywq]], [[2i7h]] – NR + FMN – ''Bacillus cereus''<br />
-
**[[5hdj]] – NR – ''Bacillus megaterium''<br />
+
**[[5hdj]] – NR + FMN – ''Bacillus megaterium''<br />
-
**[[2b67]] – NR – ''Streptococcus pneumonia''<br />
+
**[[2b67]] – NR + FMN – ''Streptococcus pneumonia''<br />
-
**[[3ek3]], [[3hoi]] – NR – ''Bacterioides fragilis''<br />
+
**[[3ek3]], [[3hoi]] – NR + FMN – ''Bacterioides fragilis''<br />
**[[3g14]] – NR – ''Clostridium novyi''<br />
**[[3g14]] – NR – ''Clostridium novyi''<br />
-
**[[3h4o]], [[3koq]] - NR – ''Clostridium difficile''<br />
+
**[[3h4o]], [[3koq]] - NR + FMN – ''Clostridium difficile''<br />
-
**[[3gr3]] – NR – ''Bartonella henselae''<br />
+
**[[3gr3]] – NR + FMN – ''Bartonella henselae''<br />
**[[3hzn]] – NR – ''Salmonella typhimurium''<br />
**[[3hzn]] – NR – ''Salmonella typhimurium''<br />
-
**[[3k6h]] – NR – ''Agrobacterium tumefaciens''<br />
+
**[[3k6h]] – NR + FMN – ''Agrobacterium tumefaciens''<br />
-
**[[2wzv]], [[2ymv]] – MsNR – ''Mycobacterium smegmatis''<br />
+
**[[2wzv]], [[2ymv]] – MsNR + FMN – ''Mycobacterium smegmatis''<br />
-
**[[3of4]] – NR FMN/FAD – ''Idiomarina loihiensis''<br />
+
**[[3of4]] – NR + FMN + FAD – ''Idiomarina loihiensis''<br />
-
**[[3n2s]] – NR – ''Bacillus subtilis''<br />
+
**[[3n2s]] – NR + FMN – ''Bacillus subtilis''<br />
-
**[[3pxv]] – NR – ''Desulfitobacterium hafniense''<br />
+
**[[3pxv]] – NR + FMN – ''Desulfitobacterium hafniense''<br />
-
**[[3qdl]] – NR – ''Helicobacter pylori''<br />
+
**[[3qdl]] – NR + FMN – ''Helicobacter pylori''<br />
**[[3r5l]], [[3r5p]] - MtNR – ''Mycobacterium tuberculosis''<br />
**[[3r5l]], [[3r5p]] - MtNR – ''Mycobacterium tuberculosis''<br />
-
**[[3r5r]], [[3r5w]] – MtNR F420<br />
+
**[[3ge6]] – NR + FMN – ''Exiguobacterium sibiricum''<br />
-
**[[3ge6]] – NR – ''Exiguobacterium sibiricum''<br />
+
**[[4dn2]] – NR + FMN – ''Geobacter metallireducens''<br />
-
**[[4dn2]] – NR – ''Geobacter metallireducens''<br />
+
**[[5ko7]], [[5ko8]], [[5krd]] – NR + FMN – ''Haliscomenobacter hydrossis''<br />
-
**[[5ko7]], [[5ko8]], [[5krd]] – NR – ''Haliscomenobacter hydrossis''<br />
+
**[[7dp0]] – SpNR + FMN – ''Sphingopyxis''<br />
 +
**[[7dp1]], [[7dp2]] – SpNR (mutant) + FMN <br />
 +
**[[6czp]] – NR + FMN – ''Vibrio vulnificus''<br />
*Copper-induced nitroreductase
*Copper-induced nitroreductase
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**[[2wqf]] – LlNR – ''Lactococcus lactis''<br />
+
**[[2wqf]] – LlNR + FMN – ''Lactococcus lactis''<br />
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**[[4bn6]] – LlNR + chloramphenicol<br />
+
**[[4bn6]] – LlNR + FMN + chloramphenicol<br />
-
**[[4bn7]], [[4bn8]] – LlNR + phenol derivative<br />
+
**[[4bn7]], [[4bn8]] – LlNR + FMN + phenol derivative<br />
-
**[[4bn9]] – LlNR + nicotinic acid<br />
+
**[[4bn9]] – LlNR + FMN + nicotinic acid<br />
-
**[[4bnb]] – LlNR + nitroquinoline<br />
+
**[[4bnb]] – LlNR + FMN + nitroquinoline<br />
*Nitrobenzoate nitroreductase
*Nitrobenzoate nitroreductase
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**[[4z85]] – NR – ''Psudomonas fluorescens''<br />
**[[4z85]] – NR – ''Psudomonas fluorescens''<br />
-
*Nitroreductase binary complex
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*Nitroreductase complex
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**[[1kqb]], [[5j8g]]– EclNR + benzoate derivative<br />
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**[[1kqb]], [[5j8g]]– EclNR + FMN + benzoate derivative<br />
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**[[1kqc]] – EclNR + acetate<br />
+
**[[1kqc]] – EclNR + FMN + acetate<br />
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**[[1ylr]], [[1ylu]] - EcNR + acetate<br />
+
**[[1ylr]], [[1ylu]] - EcNR + FMN + acetate<br />
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**[[1icr]], [[1icu]], [[1icv]], [[5j8d]] – EcNR + NAD derivative<br />
+
**[[1icr]], [[1icu]], [[1icv]], [[5j8d]] – EcNR + FMN + NAD derivative<br />
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**[[1ooq]] – EcNR + dicoumarol<br />
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**[[1ooq]] – EcNR + FMN + dicoumarol<br />
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**[[1oo6]], [[1oon]], [[1idt]] – EcNR + benzamide derivative<br />
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**[[1oo6]], [[1oon]], [[1idt]] – EcNR + FMN + benzamide derivative<br />
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**[[1yki]] – EcNR + antibiotic<br />
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**[[1yki]] – EcNR + FMN + antibiotic<br />
-
**[[2wzw]] - MsNR + NADPH<br />
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**[[7nb9]] – EcNR + FMN + nitrofurantoin<br />
 +
**[[7nmp]], [[7nnx]] – EcNR + FMN + quinone derivative<br />
 +
**[[7niy]] – EcNR (mutant) + FMN + imidazole<br />
 +
**[[2wzw]] - MsNR + FMN + NADPH<br />
**[[6czp]] – NR + FMN – ''Vibrio vulnificus''<br />
**[[6czp]] – NR + FMN – ''Vibrio vulnificus''<br />
 +
**[[3r5r]], [[3r5w]] – MtNR F420<br />
}}
}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Revision as of 08:06, 24 November 2021

E. coli nitroreductase dimer containing FMN and nicotinic acid (PDB entry 1icr)

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3D structures of nitroreductase

Updated on 24-November-2021

References

  1. ↑ Lovering AL, Hyde EI, Searle PF, White SA. The structure of Escherichia coli nitroreductase complexed with nicotinic acid: three crystal forms at 1.7 A, 1.8 A and 2.4 A resolution. J Mol Biol. 2001 May 25;309(1):203-13. PMID:11491290 doi:10.1006/jmbi.2001.4653
  2. ↑ Williams EM, Little RF, Mowday AM, Rich MH, Chan-Hyams JV, Copp JN, Smaill JB, Patterson AV, Ackerley DF. Nitroreductase gene-directed enzyme prodrug therapy: insights and advances toward clinical utility. Biochem J. 2015 Oct 15;471(2):131-53. doi: 10.1042/BJ20150650. PMID:26431849 doi:http://dx.doi.org/10.1042/BJ20150650

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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