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2bw3

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Revision as of 13:19, 24 November 2021

Three-dimensional structure of the Hermes DNA transposase

Structural highlights

2bw3 is a 2 chain structure with sequence from House fly. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Mobile elements and their inactive remnants account for large proportions of most eukaryotic genomes, where they have had central roles in genome evolution. Over 50 years ago, McClintock reported a form of stress-induced genome instability in maize in which discrete DNA segments move between chromosomal locations. Our current mechanistic understanding of enzymes catalyzing transposition is largely limited to prokaryotic transposases. The Hermes transposon from the housefly is part of the eukaryotic hAT superfamily that includes hobo from Drosophila, McClintock's maize Activator and Tam3 from snapdragon. We report here the three-dimensional structure of a functionally active form of the transposase from Hermes at 2.1-A resolution. The Hermes protein has some structural features of prokaryotic transposases, including a domain with a retroviral integrase fold. However, this domain is disrupted by the insertion of an additional domain. Finally, transposition is observed only when Hermes assembles into a hexamer.

Molecular architecture of a eukaryotic DNA transposase.,Hickman AB, Perez ZN, Zhou L, Musingarimi P, Ghirlando R, Hinshaw JE, Craig NL, Dyda F Nat Struct Mol Biol. 2005 Aug;12(8):715-21. Epub 2005 Jul 24. PMID:16041385^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hickman AB, Perez ZN, Zhou L, Musingarimi P, Ghirlando R, Hinshaw JE, Craig NL, Dyda F. Molecular architecture of a eukaryotic DNA transposase. Nat Struct Mol Biol. 2005 Aug;12(8):715-21. Epub 2005 Jul 24. PMID:16041385 doi:10.1038/nsmb970

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bw3"

@@ Line 3: / Line 3: @@
 <StructureSection load='2bw3' size='340' side='right'caption='[[2bw3]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2bw3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/House_fly House fly]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BW3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2bw3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/House_fly House fly]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BW3 FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bw3 OCA], [http://pdbe.org/2bw3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bw3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bw3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bw3 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bw3 OCA], [https://pdbe.org/2bw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bw3 RCSB], [https://www.ebi.ac.uk/pdbsum/2bw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bw3 ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 28: / Line 28: @@
 ==See Also==
-*[[Transposase|Transposase]]
+*[[Transposase 3D structures|Transposase 3D structures]]
 == References ==
 <references/>

2bw3

From Proteopedia

Revision as of 13:19, 24 November 2021

Three-dimensional structure of the Hermes DNA transposase

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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