7n75
From Proteopedia
(Difference between revisions)
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==Cryo-EM structure of ATP13A2 D458N/D962N mutant in the E1-apo state, Conformation 1== | ==Cryo-EM structure of ATP13A2 D458N/D962N mutant in the E1-apo state, Conformation 1== | ||
| - | <StructureSection load='7n75' size='340' side='right'caption='[[7n75]]' scene=''> | + | <StructureSection load='7n75' size='340' side='right'caption='[[7n75]], [[Resolution|resolution]] 2.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N75 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7n75]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N75 FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n75 OCA], [https://pdbe.org/7n75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n75 RCSB], [https://www.ebi.ac.uk/pdbsum/7n75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n75 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n75 OCA], [https://pdbe.org/7n75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n75 RCSB], [https://www.ebi.ac.uk/pdbsum/7n75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n75 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Polyamines are small, organic polycations that are ubiquitous and essential to all forms of life. Currently, how polyamines are transported across membranes is not understood. Recent studies have suggested that ATP13A2 and its close homologs, collectively known as P5B-ATPases, are polyamine transporters at endo-/lysosomes. Loss-of-function mutations of ATP13A2 in humans cause hereditary early-onset Parkinson's disease. To understand the polyamine transport mechanism of ATP13A2, we determined high-resolution cryoelectron microscopy (cryo-EM) structures of human ATP13A2 in five distinct conformational intermediates, which together, represent a near-complete transport cycle of ATP13A2. The structural basis of the polyamine specificity was revealed by an endogenous polyamine molecule bound to a narrow, elongated cavity within the transmembrane domain. The structures show an atypical transport path for a water-soluble substrate, in which polyamines may exit within the cytosolic leaflet of the membrane. Our study provides important mechanistic insights into polyamine transport and a framework to understand the functions and mechanisms of P5B-ATPases. | ||
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| + | Structural basis of polyamine transport by human ATP13A2 (PARK9).,Sim SI, von Bulow S, Hummer G, Park E Mol Cell. 2021 Oct 21. pii: S1097-2765(21)00684-5. doi:, 10.1016/j.molcel.2021.08.017. PMID:34715013<ref>PMID:34715013</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7n75" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Park E]] | + | [[Category: Park, E]] |
| - | [[Category: Sim | + | [[Category: Sim, S I]] |
| + | [[Category: P-type atpase]] | ||
| + | [[Category: P5b-atpase]] | ||
| + | [[Category: Polyamine transporter]] | ||
| + | [[Category: Transport protein]] | ||
Revision as of 06:30, 1 December 2021
Cryo-EM structure of ATP13A2 D458N/D962N mutant in the E1-apo state, Conformation 1
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