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1fuu
From Proteopedia
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[[Image:1fuu.jpg|left|200px]] | [[Image:1fuu.jpg|left|200px]] | ||
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'''YEAST INITIATION FACTOR 4A''' | '''YEAST INITIATION FACTOR 4A''' | ||
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[[Category: Johnson, E R.]] | [[Category: Johnson, E R.]] | ||
[[Category: McKay, D B.]] | [[Category: McKay, D B.]] | ||
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| - | [[Category: | + | [[Category: Helicase]] |
| - | [[Category: | + | [[Category: If4a]] |
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Revision as of 13:47, 2 May 2008
YEAST INITIATION FACTOR 4A
Overview
The eukaryotic translation initiation factor 4A (eIF4A) is a member of the DEA(D/H)-box RNA helicase family, a diverse group of proteins that couples an ATPase activity to RNA binding and unwinding. Previous work has provided the structure of the amino-terminal, ATP-binding domain of eIF4A. Extending those results, we have solved the structure of the carboxyl-terminal domain of eIF4A with data to 1.75 A resolution; it has a parallel alpha-beta topology that superimposes, with minor variations, on the structures and conserved motifs of the equivalent domain in other, distantly related helicases. Using data to 2.8 A resolution and molecular replacement with the refined model of the carboxyl-terminal domain, we have completed the structure of full-length eIF4A; it is a "dumbbell" structure consisting of two compact domains connected by an extended linker. By using the structures of other helicases as a template, compact structures can be modeled for eIF4A that suggest (i) helicase motif IV binds RNA; (ii) Arg-298, which is conserved in the DEA(D/H)-box RNA helicase family but is absent from many other helicases, also binds RNA; and (iii) motifs V and VI "link" the carboxyl-terminal domain to the amino-terminal domain through interactions with ATP and the DEA(D/H) motif, providing a mechanism for coupling ATP binding and hydrolysis with conformational changes that modulate RNA binding.
About this Structure
1FUU is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase., Caruthers JM, Johnson ER, McKay DB, Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):13080-5. PMID:11087862 Page seeded by OCA on Fri May 2 16:47:30 2008
