2d5v

From Proteopedia

(Difference between revisions)

Revision as of 10:27, 8 December 2021

Crystal structure of HNF-6alpha DNA-binding domain in complex with the TTR promoter

Structural highlights

2d5v is a 6 chain structure with sequence from Buffalo rat. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HNF6_RAT] Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. The affinity of HNF-6-alpha and HNF-6-beta for DNA differs depending on the target sequence.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Hepatocyte nuclear factor-6 (HNF-6), a liver-enriched transcription factor, controls the development of various tissues, such as the pancreas and liver, and regulates the expression of several hepatic genes. This protein belongs to the ONECUT class of homeodomain proteins and contains a bipartite DNA-binding domain composed of a single cut domain and a characteristic homeodomain. This transcription factor has two distinct modes of DNA binding and transcriptional activation that use different coactivators depending on the target gene. The crystal structure of the bipartite DNA-binding domain of HNF-6alpha complexed with the HNF-6-binding site of the TTR promoter revealed the DNA recognition mechanism of this protein. Comparing our structure with the DNA-free structure of HNF-6 or the structure of Oct-1, we discuss characteristic features associated with DNA binding and the structural basis for the dual mode of action of this protein, and we suggest a strategy for variability of transcriptional activation of the target gene.

DNA recognition mechanism of the ONECUT homeodomain of transcription factor HNF-6.,Iyaguchi D, Yao M, Watanabe N, Nishihira J, Tanaka I Structure. 2007 Jan;15(1):75-83. PMID:17223534^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Iyaguchi D, Yao M, Watanabe N, Nishihira J, Tanaka I. DNA recognition mechanism of the ONECUT homeodomain of transcription factor HNF-6. Structure. 2007 Jan;15(1):75-83. PMID:17223534 doi:10.1016/j.str.2006.11.004

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2d5v"

@@ Line 3: / Line 3: @@
 <StructureSection load='2d5v' size='340' side='right'caption='[[2d5v]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2d5v]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D5V FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2d5v]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D5V FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d5v OCA], [http://pdbe.org/2d5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d5v RCSB], [http://www.ebi.ac.uk/pdbsum/2d5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2d5v ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d5v OCA], [https://pdbe.org/2d5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d5v RCSB], [https://www.ebi.ac.uk/pdbsum/2d5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d5v ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/HNF6_RAT HNF6_RAT]] Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. The affinity of HNF-6-alpha and HNF-6-beta for DNA differs depending on the target sequence.
+[[https://www.uniprot.org/uniprot/HNF6_RAT HNF6_RAT]] Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. The affinity of HNF-6-alpha and HNF-6-beta for DNA differs depending on the target sequence.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

2d5v

From Proteopedia

Revision as of 10:27, 8 December 2021

Crystal structure of HNF-6alpha DNA-binding domain in complex with the TTR promoter

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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