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1fw8
From Proteopedia
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[[Image:1fw8.gif|left|200px]] | [[Image:1fw8.gif|left|200px]] | ||
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'''CIRCULARLY PERMUTED PHOSPHOGLYCERATE KINASE FROM YEAST: PGK P72''' | '''CIRCULARLY PERMUTED PHOSPHOGLYCERATE KINASE FROM YEAST: PGK P72''' | ||
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[[Category: Ritco-Vonsovici, M.]] | [[Category: Ritco-Vonsovici, M.]] | ||
[[Category: Tougard, P.]] | [[Category: Tougard, P.]] | ||
| - | [[Category: | + | [[Category: Glycolysis]] |
| - | [[Category: | + | [[Category: Kinase]] |
| - | [[Category: | + | [[Category: Mutant]] |
| - | [[Category: | + | [[Category: Permutation]] |
| - | [[Category: | + | [[Category: Permuted sequence]] |
| - | [[Category: | + | [[Category: Pgk]] |
| - | [[Category: | + | [[Category: Phosphoglycerate kinase]] |
| - | [[Category: | + | [[Category: Phosphotransferase]] |
| - | [[Category: | + | [[Category: Protein folding,two-domain protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:50:10 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:50, 2 May 2008
CIRCULARLY PERMUTED PHOSPHOGLYCERATE KINASE FROM YEAST: PGK P72
Overview
The crystallographic structure of a circularly permuted form of yeast PGK, 72p yPGK, has been determined to a resolution of 2.3 A by molecular replacement. In this engineered protein, the C- and N-terminal residues of the wild-type protein are directly connected by a peptide bond and new N- and C-terminal residues are located within the N-terminal domain. The overall fold of the protein is very similar to that of the wild-type protein, directly demonstrating that the continuity of a folding unit is not relevant to the folding process of the whole protein. Only limited structural changes were observed: these were in the regions associated with the new connection, in a long flexible loop in the permuted domain and in the vicinity of Arg38, a functionally important residue. The relative positions of the two domains suggested that this permuted protein adopts one of the most open/twisted conformations seen amongst PGKs of known structure. The effect of the mutation on the functional properties is more easily accounted for by a restriction of hinge-bending motion than by structural changes in the protein.
About this Structure
1FW8 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of a circularly permuted phosphoglycerate kinase., Tougard P, Bizebard T, Ritco-Vonsovici M, Minard P, Desmadril M, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2018-23. Epub 2002, Nov 23. PMID:12454459 Page seeded by OCA on Fri May 2 16:50:10 2008
