1fwi

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[[Image:1fwi.gif|left|200px]]
[[Image:1fwi.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1fwi |SIZE=350|CAPTION= <scene name='initialview01'>1fwi</scene>, resolution 2.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1fwi", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=ACT:Residue+Implicated+In+Catalysis'>ACT</scene> and <scene name='pdbsite=NIL:Ni+Metallocenter'>NIL</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1fwi| PDB=1fwi | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fwi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fwi OCA], [http://www.ebi.ac.uk/pdbsum/1fwi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fwi RCSB]</span>
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}}
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'''KLEBSIELLA AEROGENES UREASE, H134A VARIANT'''
'''KLEBSIELLA AEROGENES UREASE, H134A VARIANT'''
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[[Category: Karplus, P A.]]
[[Category: Karplus, P A.]]
[[Category: Pearson, M A.]]
[[Category: Pearson, M A.]]
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[[Category: hydrolase(urea amido)]]
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[[Category: Mutant]]
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[[Category: mutant]]
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[[Category: Nickel metalloenzyme]]
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[[Category: nickel metalloenzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:50:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:31:39 2008''
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Revision as of 13:50, 2 May 2008

Image:1fwi.gif

Template:STRUCTURE 1fwi

KLEBSIELLA AEROGENES UREASE, H134A VARIANT


Overview

A mutant form of Klebsiella aerogenes urease possessing Ala instead of His at position 134 (H134A) is inactive and binds approximately half the normal complement of nickel (Park, I.-S., and Hausinger, R. P.(1993) Protein Sci. 2, 1034-1041). The crystal structure of the H134A protein was obtained at 2.0-A resolution, and it confirms that only Ni-1 of the two nickel ions found in the native enzyme is present. In contrast to the pseudotetrahedral geometry observed for Ni-1 in native urease (where it is liganded by His-246, His-272, one oxygen atom of carbamylated Lys-217, and a water molecule at partial occupancy), the mononickel metallocenter in the H134A protein was found to possess octahedral geometry and was coordinated by the above protein ligands plus three water molecules. The nickel site of H134A urease was probed by UV-visible, variable temperature magnetic circular dichroism, and x-ray absorption spectroscopies. The spectroscopic data are consistent with the presence of Ni(II) in octahedral geometry coordinated by two histidylimidazoles and additional oxygen and/or nitrogen donors. These data underscore the requirement of Ni-2 for formation of active urease and demonstrate the important role of Ni-2 in establishing the proper Ni-1 coordination geometry.

About this Structure

1FWI is a Protein complex structure of sequences from Klebsiella aerogenes. Full crystallographic information is available from OCA.

Reference

Characterization of the mononickel metallocenter in H134A mutant urease., Park IS, Michel LO, Pearson MA, Jabri E, Karplus PA, Wang S, Dong J, Scott RA, Koehler BP, Johnson MK, Hausinger RP, J Biol Chem. 1996 Aug 2;271(31):18632-7. PMID:8702515 Page seeded by OCA on Fri May 2 16:50:46 2008

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OCA

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