7pq2
From Proteopedia
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==Crystal Structure of the Ring Nuclease 0811 from Sulfolobus islandicus (Sis0811) in its apo form== | ==Crystal Structure of the Ring Nuclease 0811 from Sulfolobus islandicus (Sis0811) in its apo form== | ||
| - | <StructureSection load='7pq2' size='340' side='right'caption='[[7pq2]]' scene=''> | + | <StructureSection load='7pq2' size='340' side='right'caption='[[7pq2]], [[Resolution|resolution]] 2.38Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PQ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PQ2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7pq2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PQ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PQ2 FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pq2 OCA], [https://pdbe.org/7pq2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pq2 RCSB], [https://www.ebi.ac.uk/pdbsum/7pq2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pq2 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pq2 OCA], [https://pdbe.org/7pq2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pq2 RCSB], [https://www.ebi.ac.uk/pdbsum/7pq2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pq2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Type III CRISPR-Cas effector systems detect foreign RNA triggering DNA and RNA cleavage and synthesizing cyclic oligoadenylate molecules (cA) in their Cas10 subunit. cAs act as a second messenger activating auxiliary nucleases, leading to an indiscriminate RNA degradation that can end in cell dormancy or death. Standalone ring nucleases are CRISPR ancillary proteins which downregulate the strong immune response of Type III systems by degrading cA. These enzymes contain a CRISPR-associated Rossman-fold (CARF) domain, which binds and cleaves the cA molecule. Here, we present the structures of the standalone ring nuclease from Sulfolobus islandicus (Sis) 0811 in its apo and post-catalytic states. This enzyme is composed by a N-terminal CARF and a C-terminal wHTH domain. Sis0811 presents a phosphodiester hydrolysis metal-independent mechanism, which cleaves cA4 rings to generate linear adenylate species, thus reducing the levels of the second messenger and switching off the cell antiviral state. The structural and biochemical analysis revealed the coupling of a cork-screw conformational change with the positioning of key catalytic residues to proceed with cA4 phosphodiester hydrolysis in a non-concerted manner. | ||
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| + | Structural basis of cyclic oligoadenylate degradation by ancillary Type III CRISPR-Cas ring nucleases.,Molina R, Jensen ALG, Marchena-Hurtado J, Lopez-Mendez B, Stella S, Montoya G Nucleic Acids Res. 2021 Dec 2;49(21):12577-12590. doi: 10.1093/nar/gkab1130. PMID:34850143<ref>PMID:34850143</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7pq2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Jensen | + | [[Category: Jensen, A L.G]] |
| - | [[Category: Lopez-Mendez B]] | + | [[Category: Lopez-Mendez, B]] |
| - | [[Category: Marchena-Hurtado J]] | + | [[Category: Marchena-Hurtado, J]] |
| - | [[Category: Molina R]] | + | [[Category: Molina, R]] |
| - | [[Category: Montoya G]] | + | [[Category: Montoya, G]] |
| - | [[Category: Stella S]] | + | [[Category: Stella, S]] |
| + | [[Category: Antiviral protein]] | ||
| + | [[Category: Carf nucleotide-binding domain]] | ||
| + | [[Category: Crispr-associated protein]] | ||
| + | [[Category: Helix-turn-helix]] | ||
| + | [[Category: Ring nuclease]] | ||
| + | [[Category: Viral resistance]] | ||
Revision as of 17:01, 15 December 2021
Crystal Structure of the Ring Nuclease 0811 from Sulfolobus islandicus (Sis0811) in its apo form
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